ABSTRACT
Objective To explore the risk factors of type 2 diabetes mellitus(T2DM)in mountainous countryside residents in order to provide relevant intervention measures.Methods 80 new cases of T2DM were selected from pingyuan community in Liandu District of Lishui City as case group,and 1 60 normal people were selected as control group at the same time and places,in which the 1∶2 matched design of case -control study method was used.Conditional Logistic regression analysis was applied in this study.Results The multiple conditional logistic regression analysis suggested that primary family history,history of hypertension,abdominal obesity were the risk factors of T2DM(OR=2.08,1.55 and 2.78 respectively);while education level,exercise and fish intake were the protective factors(OR=0.74,0.59 and 0.32 respectively).Conclusion T2DM is the result of both genetic factors and environmental factors.Moreover,health education,balanced diet and proper physical exercise should be encouraged among residents to prevent T2DM in mountainous countryside.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between cell apoptosis and the quality of early mouse embryos, understand the significance of apoptosis-regulatory genes in early embryonic development, and explore a new approach to improving the embryo quality.</p><p><b>METHODS</b>The levels of cell apoptosis and proliferation in early mouse embryos in different developmental status (morphologically normal embryos, arrested embryos and fragmented embryos) were analyzed with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), caspase in situ fluorescence and Bcl-2 immunofluorescence, and immunofluorescent detection of proliferating cell nuclear antigen (PCNA).</p><p><b>RESULTS</b>The cells in arrested embryos and embryonic fragments showed positive results in TUNEL assay with enhanced caspase activity and lowered expressions of Bcl-2 and PCNA.</p><p><b>CONCLUSION</b>Cell apoptosis in early mouse embryos may be closely related to embryonic arrest and fragmentation.</p>
Subject(s)
Animals , Female , Mice , Pregnancy , Apoptosis , Caspases , Metabolism , Embryo, Mammalian , Cell Biology , Mice, Inbred Strains , Proliferating Cell Nuclear Antigen , Metabolism , Proto-Oncogene Proteins , Metabolism , Proto-Oncogene Proteins c-bcl-2ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the value of endometrial microvessel density (MVD) in assessing the endometrial receptivity during the peri-implantation period.</p><p><b>METHODS</b>A total of 104 patients undergoing in vitro fertilization and embryo transfer (IVF-ET) treatment were analyzed retrospectively. The subjects were divided into clinical pregnancy group (50 cases) and nonpregnant group (54 cases) according to the IVF-ET outcome. Endometrial tissues were collected 7 days after the natural ovulation prior to IVF-ET for measurement of the endometrial MVD using electron microscopy, which was analyzed in relation to the clinical outcome of the treatment.</p><p><b>RESULTS</b>The endometrial MVD was significantly higher in the clinical pregnancy group than in the nonpregnant group [(4.12∓1.84)% vs (3.46∓1.26)%, t=-2.127, P=0.036). ROC curve analysis showed that the MVD had an area under the curve slightly over 0.5 (0.598) for predicting clinical pregnancy, suggesting a poor specificity in predicting the clinical outcome of the treatment.</p><p><b>CONCLUSION</b>In IVF-ET cycles, the endometrial MVD during the peri-implantation period is helpful for assessing the endometrial receptivity, but the specificity remains low.</p>
Subject(s)
Adult , Female , Humans , Embryo Implantation , Physiology , Embryo Transfer , Endometrium , Physiology , Fertilization in Vitro , Infertility, Female , Diagnostic Imaging , Therapeutics , Microvessels , Retrospective Studies , UltrasonographyABSTRACT
<p><b>OBJECTIVE</b>To study the clinical factors affecting the outcomes of repeated assisted reproductive technology (ART) cycles.</p><p><b>METHODS</b>A retrospective analysis of the clinical data and outcomes was conducted among 160 patients undergoing repeated IVF/ICSI-ET treatment between January 2006 and April 2009.</p><p><b>RESULTS</b>The patients with successful clinical pregnancy after two ART cycles (group A) had a younger age and shorter duration of infertility, and had more antral follicles (AFC), more eggs and good-quality embryos with more transferred embryos available and higher good-quality embryo rate (P<0.05) than those who failed to have pregnancy after the cycles (group B). In the second cycle, the patients in group A had higher doses of short-acting GnRHa, r-HCG and HMG and at the same time more good eggs and embryos than in the first cycle.</p><p><b>CONCLUSIONS</b>Female age is one of the most important factors affecting the pregnancy rate after repeated ART cycles. The clinical pregnancy rate can be enhanced by administering short-acting GnRHa, HMG, oral contraceptives and adjusting the dose of Gn as well as changing the culture medium of embryos.</p>