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Chinese Journal of Biotechnology ; (12): 690-694, 2008.
Article in Chinese | WPRIM | ID: wpr-342849

ABSTRACT

Several antigen epitopes were designed according to the sequences of Swine influenza virus hemagglutinin (HA) genes and lined with the interval. The gene was amplified by PCR and sub cloned into pET30a (+) vector. The fusion protein was expressed in E. coli BL21 (DE3) by induced with IPTG and purified by affinity chromatography. The molecular weight of the protein was about 20 kD in SDS-PAGE. Immunological activity of the fusion protein was analyzed by Western blot. The results showed that the fusion protein could interact with anti-His antibody and the rabbit antiserum against SIV. The immunized mouse can produced antibodies against the target peptide and HI antibody against SIV H1N1 or H3N2. This study provides a new vaccine candidate for SIV.


Subject(s)
Animals , Humans , Mice , Amino Acid Sequence , Antibodies, Viral , Blood , Antigens, Viral , Genetics , Allergy and Immunology , Base Sequence , Epitopes , Genetics , Allergy and Immunology , Metabolism , Escherichia coli , Genetics , Metabolism , Hemagglutinins , Genetics , Allergy and Immunology , Immunization , Influenza A Virus, H1N1 Subtype , Genetics , Allergy and Immunology , Influenza A Virus, H3N2 Subtype , Genetics , Allergy and Immunology , Mice, Inbred BALB C , Molecular Sequence Data , Random Allocation , Recombinant Fusion Proteins , Genetics , Allergy and Immunology
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