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Objective To observe the effect of Tanggankang on the expression of CREB in diabetic-fatty liver rats;To investigate the mechanism of Tanggankang. Methods STZ and feeding high fat forage induced diabetic rats were randomly divided into model group, Huganning group, Tanggankang high dose group, Tanggankang medium dose group, Tanggankang low dose group, 10 rats in each group, with 10 normal rats as control. After 12 weeks of drug intervention, rats were killed, the livers were removed, and the expression of CREB was detected by Western blot. Results Compared with normal group, the level of CREB of model group was significantly decreased (P<0.01). Compared with model group, Tanggankang low, medium and high dose group significantly decreased the level of CREB (P<0.01). Conclusion Tanggankang may play an active role in glucose and lipid metabolism by affecting the expression of CREB.
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Objective:To observe the effect of Tangshenning on renal function and LOX-1 mRNA expression in the kidney of early diabetic nephropathy rats model. Methods:Early diabetic nephropathy rats model was made with high fat diet, STZ and unilateral ne-phregtomy. After the 12-week drug intervention, the rats were sacrificed, the kidneys were removed and the mRNA expressions of LOX-1 in the kidney were observed by the method of RT-PCR. Results:Early diabetic nephropathy rats model was successfully made by the triple-modeling method. The mRNA expression of LOX-1 in the kidney was significantly increased, and Tangshenning could low-er the expression of LOX-1 in the kidney in a dose-dependent manner. Conclusion:The abnormal expression of LOX-1 could be one of the mechanisms for diabetic nephropathy. Tangshening has good kidney protective effect through decreasing LOX-1 abnormal expression in the kidneys, which deserves further research.
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Objective To observe effects of Huanglian Jiedu Decoction on fasting blood glucose (FBG), fasting insulin (FINS), abdominal aortic morphology and expression of HIF-1α in the abdominal aorta in rats with type 2 diabetes. Methods The rat model of type 2 diabetes was established by intravenous injection of streptozotocin and feeding with high sugar and high fat diet. Rats were randomly divided into blank group, model group, control group and treatment group. The blank group and model group were given normal saline by gavage, the control group was given metformin, and the treatment group was given Huanglian Jiedu Decoction. Six weeks later, the levels of FBG and FINS were determined. Immunohistochemical method was used to determine the expression of HIF-1α in abdominal aorta and HE staining method to observe the changes of abdominal aortic morphology. Results Compared with model group and control group, abdominal aorta of HIF-1α and FBG level of treatment group were significantly decreased (P <0.01), FINS significantly increased (P <0.01), and abdominal aortic endothelial cell injury was significantly improved. Conclusion Huanglian Jiedu Decoction has a protective effect on the injury of vascular endothelium, and its mechanism may be related with inhibiting the expression of HIF-1α.
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Objective To study the effect of Tanggankang capsule treating liver trauma of diabetic mice.Methods Healthy Wistar rats are used in this trial. STZ are injected into the mice’s trail vein and they are fed with feed stuff with high quantity of heat. Eight weeks later, rats with blood sugar≥11.1mmol and disturbance of lipid metabolism are selected as diabetic model. 50 rats were randomly divided into five groups:low,moderate and high dosage groups;Metformin Hydrochloride and Glucurolactone control group。Every rat is given equal dosage by mouth once a day.After 6 weeks and 12 weeks, measure the blood sugar and lipid. At the 12th weeks, rats are sacrificed to weigh the liver and compute the liver coefficient and measure TC,LPO,ALT and AST. Results Tanggankang capsule has good therapeutic effect on treating hyperglycemia and disturbance of lipid metabolism for diabetic model; Tanggankang capsule can effectively prevent from the pathological change of diabetic fatty liver;what’s more,the more dosage and longer duration of drug,the stronger effect will appear,and the effect of large dosage is better than that of control group,thus the difference is remarkable (P
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Objective To explore the role of Tanggankang Capsule in decreasing blood sugar, regulating blood lipid and protecting liver for the patient of type 2 diabetes with hepatic injury. Methods 60 cases of type 2 diabetes with hepatic injury, and with TCM syndrome of stagnation of liver-qi and deficiency of spleen, dampness-heat and blood stasis were enrolled in the study. Patients were randomly divided into treatment group (Tanggankang Capsule group, n=30) and control group (Metfofmin and Glucurolactone group, n=30), and were administrated for 8 weeks. Results The total effective rate for the improvement of TCM syndromes was 89.9% in the treatment group and 62.4% in the control group, with significant difference between the two groups (P