The kinetic alteration of nitric oxide formation in the lungs in the development of pulmonary fibrosis of rats / 中国病理生理杂志

AIM: To observe the kinetic alteration of nitric oxide formation in the lungs in the development of pulmonary fibrosis in the rat. METHODS: The contents of hydroxyproline in the lungs, NO - 2/NO - 3 (nitrite/nitrate) in out-flowing and in-flowing pulmonary blood (OPB, IPB) were assayed on the day 7, 14, 21, 30 and 70 after intratracheal administration of bleomycin A 5 . The content of NO - 2/NO - 3 in supernatants of culture of the alveolar macrophages (AMs) and the amount of iNOS positive stain cells in lung tissue section were also observed in the rat on 14th day after-bleomycin A 5 administration. RESULTS: The content of lung hydroxyproline had no change on the 7th day, increased on the 14th day ( P 0.05) but still decreased in IPB ( P