Associations of signal transducer and activators of transcription 3 polymorphism with the susceptibility to hepatitis B virus-related hepatocellular carcinoma / 中华预防医学杂志

<p><b>OBJECTIVE</b>To evaluate the association of signal transducer and activators of transcription 3 (STAT3) -1096G/C polymorphism in promoter region with the susceptibility to HBsAg positive hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>A total of 632 patients with HCC and 723 HBV-infected subjects without HCC treated at Changhai Hospital of Shanghai from 2009 to 2012 were included in this case-control study. The polymorphism of STAT3 -1096 G/C was genotyped by Fluorescent probe-Real time quantitative PCR. Univariate analysis was used to calculate the odds ratio (OR) and its 95% confidence interval (CI).</p><p><b>RESULTS</b>The frequency of genetic allele STAT3 -1096G/C (GC+CC) of control group and case group were 61.83% (447/723) and 60.60% (383/632), while difference of HCC risk was not found among different genotypes (OR = 0.95, 95%CI: 0.76-1.18). When stratified by sex, the frequency of genetic allele STAT3 -1096C (GC+CC) of control group and case group were 62.18% (314/505) and 61.75% (331/536) in men, 61.01% (133/218) and 54.17% (52/96) in women, respectively, while difference of HCC risk was not found among different genotypes (OR = 0.98, 95%CI: 0.77-1.26; OR = 0.76, 95%CI: 0.47-1.26, respectively). When stratified by HBV genotypes, the frequency of genetic allele STAT3 -1096C (GC+CC) of control group and case group were 61.45% (110/179) and 53.13% (34/64) in HBV genotype B, 62.87% (276/439) and 60.27% (226/375) in HBV genotype C, respectively, while difference of HCC risk was not found among different genotypes (OR = 0.71, 95%CI: 0.40-1.26; OR = 0.90, 95%CI: 0.68-1.19, respectively).</p><p><b>CONCLUSION</b>STAT3 -1096G/C polymorphism was not associated with the susceptibility to HCC for the HBV-infected subjects without HCC.</p>

Research in clinical application of a special stool collector for paraplegic patients / 中国实用护理杂志

Objective To develop a special stool collector for paraplegic patients with constipation,and to observe the clinical effects. Methods 52 paraplegic patients with constipation were divided into group A(30 cases) and group B(22 cases ).Group A used type one stool collector and goup B chose type two stool collector. The time of dealing with defecation, exposure time, pollution area, the usage of urinal pad, toilet paper, clean water before and after the special toilets were observed between the two groups. Results Each indicator significantly changed after using two types of stool collectors in the two groups of patients and above were significantly decreased. Conclusions The special stool collector for the paraplegic patients with constipation can effectively avoid the soakage of the anal and buttocks skin, reduce the environment pollution of the odor, reduce labor intensity and lower care costs.

The dose-response relationship study between the quantitative morphological stereology on thyroid and different iodine doses in mice / 中华预防医学杂志

<p><b>OBJECTIVE</b>To study the dose-response relationship between the quantitative morphological stereology on thyroid and different iodine doses in mice.</p><p><b>METHODS</b>Weaning Kunming mice were randomly divided into seven groups. The mice were fed for 100 days with distilled water containing different KIO3 concentrations, i.e. 50, 250, 500, 1 000, 1 500, 2 000, and 3 000 microgram/L respectively. The 50 microgram/L (proper iodine concentration) group was control group, and the groups of 250 approximately 3 000 microgram/L were high iodine groups. The stereology parameters of thyroid follicle and follicular cavities were measured with HPIAS-1000 (High Resolution Pathological Image & word Analysis System). The stereology parameters included mean surface, volume on area, volume on circumference, specific surface, numerical density on area, spherical factor, the percentage of mean surface and mean volume of the follicular epithelial cell in thyroid follicle was further calculated.</p><p><b>RESULTS</b>Positive correlations was observed between the thyroid absolute and relative weight, goiter rate and different iodine doses. And the thyroid absolute and relative weight of mice in the 250 microgram/L group was significantly different from that in 50 microgram/L group. The goiter rate of mice in different high iodine groups was in conformity with that of epidemiological investigation. The goiter rate of mice in 500 microgram/L group was different from that in 50 microgram/L group. Positive correlations were observed between mean surface, volume on area, volume on circumference, spherical factor and iodine doses, but the negative correlations were observed between numerical density on area, specific surface, the percentage of mean surface and mean volume of the follicular epithelial cell in thyroid follicle and iodine doses.</p><p><b>CONCLUSIONS</b>When Iodine doses are between 250 approximately 3 000 microgram/L, the dose-response relationship was observed between the morphological stereology parameters of thyroid follicle and follicular cavities and iodine doses, and when the dose of iodine is 250 microgram/L, it is possible to induce colloid goiter of mice. The goiter rates of mice resulted by different high iodine doses were in conformity with that of the epidemiological investigation of people.</p>