ABSTRACT
Objective To establish an HPLC quantitative method for the content determination of baicalin in Kangdeling capsule .Methods The chromatography column was Agilent Tc-C18-WR (4 .6 mm × 250 mm ,5μm) ,and the column temper-ature was 30 ℃ .The mobile phase consisted of acetonitrile and 0 .5‰ phosphoric acid (26 ∶ 74 ) .The flow rate was 1 .0 ml/min ,and the detection wavelength was 265 nm .Results The retention time of baicalin was about 16 min .The calibra-tion equation was Y=22 114 .67 X -112 836 .7(r=0998 8)with good linearity in the range of 5 .410-108 .2 μg/ml for baicalin . The average recovery was 98 .78% while RSD were 0 .74% .Conclusion This method is simple ,time-saving and accurate which could be used to routine analysis of baicalin in Kangdeling capsule .
ABSTRACT
Objective To develop a method for determination of the rosmarinic acid in Xiaokang capsules .Methods An HPLC method was developed .The determination was performed on Agilent C18 column at 30℃ with a mobile phase composed of methanol :0 .5% formic acid (50∶50) run at a flow rate of 1 .0 ml/min .The detection wavelength was set at 330 nm and the injection volume was 10 μl .Results The linear range of rosmarinic acid concentrations was 2 .88 to 20 .1μg/ml (r=0 .999 8) . The average recovery was 99 .5% (RSD=0 .40% ,r=9) .Conclusion An assay for the determination of the rosmarinic acid in Xiaokang capsule that is simple ,rapid ,accurate and reliable has been developed .
ABSTRACT
Objective To study the pharmaceutical quality of the thermos sensitive insulin anal liquid suppository .Meth-ods Orthogonal test method was used ,the forming of a gelatum time ,gel strength ,bio-adhesive force ,viscosity ,gelation temperature were taken as the evaluation index ,the prescription of poloxamer 407 and poloxamer 188 ,the proportion of chi-tosan and pH were investigated ,in order to determine the best optimized prescription and measure what was the best choice of the gel strength ,biological adhesion force ,suppository viscosity ,gelation temperature for the final prescription of liquid sup-pository .Result The best combination of the prescription were 15% of poloxamer 407 ,25% of poloxamer 188 and 0 .4% of chitosan with the pH of 5 ± 0 .2 .Conclusion The test proved to be equitable with the good intensity and bio-adhesive force of the liquid suppository .
ABSTRACT
Objective To investigate the effect of microcireulation blood flow and altering immunity by Chuanxiongqin,ulinastain and thymosin α1 on sepsis patients.Methods 90 patients were randomly divided into 3 groups(n=30),namely ICU group,Chuanxiongqin group,ulinastain and thymosin α1 group.HLA-DR/CD14+and IL-6,TNF-α,Lac,DD were measured.Results (1)DD showed no significant difference at every time point between ICU group and ulinastain+thymosin α1 group(P＞0.05).DD decreased in Chuanxiongqin group,and was significantly different from the others on the third day.(2)Lac unchanged significantly at every time point in ICU group(P＞0.05).Lac in Chuanxiongqin group and ulinastain+thymeain α1 group tended to decrease,and was statistically different from ICU group on the second day.(3)IL-6 and TNF-α tended to increase at every time point in ICU group(P＜0.05).In ulinastain+thymosin α1 group,IL-6 and TNF-α returned to the level before treatment,HLA-DR/(D14+increased significantly,and was higher than Chuanxiongqin group and ICU group statistically.Conclusion Chuanxiongqin could ameliorate circulation;ulinastsin and thymosin α1 could depress IL-6,TNF-α.So ulinastain and thymosin α1 might protect the immunity of sepsis patients.