ABSTRACT
Objective To detect the levels of eosinophil and eotaxin in the children with virus associated wheezing diseases,and explore the role of both in virus correlation breathing occurrence and development.Methods Sixty-eight cases of children with virus associated wheezing diseases were selected as observation group,and the other 42 cases of healthy children were selected as control group.The peripheral eosinophil count and serum eotaxin level were detected and compared in observation group and control group on the acute stage and recovery stage.Results The eosinophil count in observation group on the acute stage was (1.12 ± 0.62) × 1099/L,the recovery stage was (0.34 ± 0.13) × 109/L,control group was (0.16 ± 0.07) ×109/L,the eosinophil count in observation group on the acute stage was higher than that on the recovery stage and control group,the eosinophil count in observation group on the recovery stage was higher than that in control group,there were statistically significant differences (P ＜ 0.05).The eotaxin level in observation group on the acute stage was (437.2 ±87.6) ng/L,the recovery stage was (182.4 ±56.2) ng/L,control group was (84.1 ± 39.7) ng/L,the eotaxin level in observation group on the acute stage was higher than that on the recovery stage and control group,the eotaxin level in observation group on the recovery stage was higher than that in control group,there were statistically significant differences (P ＜0.05).There were positively correlated between the eosinophil count and the eotaxin level in observation group on the acute stage,recovery stage and control group (r =0.579,0.593,0.581,P＜ 0.05).Conclusions The eosinophils and eotaxin may be involved in the children with virus associated wheezing diseases pathophysiological process,the levels of the eosinophils and eotaxin can monitor the course change,and be helpful for clinical diagnosis and treatment.
ABSTRACT
Objective To analyze the serumα1-antitrypsin (α1-AT) and α1-glycoprotein (α 1-AGP) in children with Wilms tumor,and to explore the clinical application value.Methods Thirtyeight children with Wilms tumor who received treatment from January 2007 to December 2010 were designed as observation group,38 healthy children were designed as control group.Fluorescence quantitative PCR was performed to analyze α 1-AT andα 1-AGP mRNA transcription levels of two groups,and ELISA was used to measure the serum concentration of α 1-AT and α 1-AGP.Results The mRNA levels of α 1-AT and α 1-AGP in observation group were 3.50 ± 0.37 and 2.10 ± 0.41,which were significantly higher than those in control group (0.90 ± 0.45,0.50 ± 0.24) (P =0.000,0.002).The levels of α 1-AT and α 1-AGP in observation group were (4516.8 ± 102.4),(1316.0 ± 27.3) g/L,which were significantly higher than those in control group [(2467.4 ± 23.8),(728.6 ± 9.4) g/L] (P =0.015,0.008).Conclusion To detect the levels of α 1-AT and α 1-AGP have the potential value for the diagnosis of Wilms tumor,which can be applied to early screening.
ABSTRACT
Objective To investigate the expression of serum vascular endothelial growth factor (VEGF) in children with Wilms tumor,and clarify the effect of VEGF in the invasion and progression of Wilms tumor.Methods Thirty children with Wilms tumor who underwent surgical treatment were retrospectively analyzed as Wilms tumor group,the control group was 30 children who underwent treatment by cold and fever symptoms.The serum VEGF levels of 2 groups were detected by enzyme-linked immunosorbent assay.Results The serum VEGF level of Wilms tumor group was significantly higher than that of control group [(567.90 ± 189.30) ng/L vs.(55.46 ± 18.49) ng/L,t =14.757,P ＜0.05].The expression of serum VEGF was closely related to NWTS stage,prognosis tissue type and lymph node metastasis (P ＜ 0.01).The expressions of serum VEGF in the NWTS Ⅲ-Ⅳ stage,lymph node metastasis and poor prognosis tissue type Wilms tumor were significantly higher than those of NWTS Ⅰ-Ⅱ stage,without lymph node metastasis,good prognosis tissue type Wilms tumor (P＜ 0.01).Conclusions The expression of serum VEGF is high in children with Wilms tumor,the serum VEGF level is closely related to NWTS stage,prognosis tissue type and lymph node metastasis.The high expression of serum VEGF shows the invasion,progression and poor prognosis in children with Wilms tumor.