ABSTRACT
The purpose of this study was to observe whether human peripheral dervied monouncleas cells (hMNCs) could participate in the regeneration process of the ischemic hearts in the way of differentiating into cardiomyocytes, vascular endothelial cells and smooth muscle cells. hMNCs were transplanted into the bodies of the mice with myocardial infarction through the tail vein injection. Hearts were harvested 2-12 weeks after injection then sliced up into frozen sections of 5 micron thickness. Double immunofluorescence staining was used to test the differentiation of the grafted cells into cardiomyocytes, smooth muscle cells and vascular endothelial cells which revealed that cells expressing both HLA and TNT, HLA and alpha-SMA, HLA and vWF existed in the hearts of the mice. According to the study, it is probable that hMNCs could participate in the regeneration process of the infarcted hearts in the way of differentiation.
Subject(s)
Animals , Humans , Mice , Cell Differentiation , Physiology , Leukocytes, Mononuclear , Transplantation , Mice, Nude , Myocardial Infarction , Pathology , Therapeutics , Myocytes, Cardiac , Cell Biology , Transplantation, HeterologousABSTRACT
Objective To observe the change of the levels of plasma anti-Ⅹa,anti-Ⅱa in acute coronary syndromes and the influence of low-molecular-weight heparin(LMWH)on plasma anti-Ⅹa and anti-Ⅱa levels.Methods Plasma anti-Ⅹa and anti-Ⅱa were measured with the specific chromogenic substrate in 24 patients with ACS and 10 healthy controls from Jan.2003 to Sep.2003.LMWHs were administrated to the ACS patients.Plasma anti-Ⅹa and anti-Ⅱa were measured immediately,and at 4 h,24 h,and day 7 after initial injection.Results Plasma anti-Ⅹa and anti-Ⅱa levels in ACS patients were significantly higher than those in controls(P