The Effect of Combining bcr-abl Antisense Phosphorothioate Oligodeoynucleotides and c-myb Aspo on K562 Cell Line / 中国肿瘤生物治疗杂志

Objective: To study the effect of combining bcr-abl Aspo and c-myb Aspo on K562 cells. Methods: Cellswere exposed to oligomers. Cell inhibitory rate was determined by typan blue dye exclusion. CFU-K562 cells were culturedin 0.8% methyleellulose. P210 was measured by flow cytometry. Cellular bcr-abl mRNA was detected by RT-PCR semiquantitative analysis. Cell apoptosis was measured by flow cytometry and observed by electron microscope. Results: When the concentration of both bcr-abl Aspo and c-myb Aspo was 5 μmol/L, K562 cells were still growth in clone state. The growth inhibitory rate was 61.7% at 120 h. P210 was depressed at 24 h and went up to 25.7% at 120 h. The apoptosis rate was 22.5%. While K562 cells were dealt with 10 μmol/L bcr-abl Aspo and 10 μmol/L c-myb Aspo, the cells were growth in dispersal. The cell growth inhibitory rate reached to 92.2% and 64.3% of K562 cells were induced to apoptosisat 120 h. P210 was complelely depressed untill 120 h. The decrease of bcr-abl mRNA was from 69.2% to 85.3% after incubation 48 h with 5 μmol/L Aspo and 10 mol/L. Conclusion: It emerges coordination to combine bcr-abl Aspo and c-myb Aspo on K562 cells, and enhances the anti-leukemia effect.

The Effect of Combining bcr-abl Antisense Phosphorothioate Oligodeoxynucleotides and c-myb Aspo on K562 Cell Line / 中国肿瘤生物治疗杂志

Objective: To study the effect of combining bcr abl Aspo and c myb Aspo on K562 cells. Methods: Cells were exposed to oligomers. Cell inhibitory rate was determined by typan blue dye exclusion. CFU K562 cells were cultured in 0.8% methylcellulose. P210 was measured by flow cytometry. Cellular bcr abl mRNA was detected by RT PCR semiquantitative analysis. Cell apoptosis was measured by flow cytometry and observed by electron microscope. Results: When the concentration of both bcr abl Aspo and c myb Aspo was 5 ?mol/L, K562 cells were still growth in clone state. The growth inhibitory rate was 61 7% at 120 h. P210 was depressed at 24 h and went up to 25.7% at 120 h. The apoptosis rate was 22.5%. While K562 cells were dealt with 10 ?mol/L bcr abl Aspo and 10 ?mol/L c myb Aspo, the cells were growth in dispersal. The cell growth inhibitory rate reached to 92.2% and 64.3% of K562 cells were induced to apoptosis at 120 h. P210 was complelely depressed untill 120 h. The decrease of bcr abl mRNA was from 69.2% to 85.3% after incubation 48 h with 5 ?mol/L Aspo and 10 ?mol/L. Conclusion: It emerges coordination to combine bcr abl Aspo and c myb Aspo on K562 cells, and enhances the anti leukemia effect.

The effect of antisense bcr - abl oligonucleotides on the growth of K562 cells / 中国病理生理杂志

AIM:To study the effect of bcr - abl gene antisense phosphorothioate oligonucleotides(Aspo) on K562 cell line and explore its significance in chronic myelogencous leukemia (CML) gene therapy. METHODS: Cells were exposed to oligomers, observed by inverted microscope. Cells inhibitory rate were determined by 0.4% trypan blue exclusion. CFU - K562 were cultured in 0.8% methylcellulose. P210 was measured by flow cytometry. RESULTS:K562 cells were treated with Aspo, they still grew in clone state and show antisense sequence specific and dose dependent. When the concentration of Aspo was more than 5?mol/L, the growth of cells was inhibited and P210 was down regulated or completely suppressed, and the greatest growth inhibition was at 120h. There was significant inhibition of cell proliferation in a rang of cells number from 1 ? 10-4/mL to 5 ? 10-4/mL after treatment with 10?mol/L Aspo. b2a2 Aspo was also effect on K562 cells which expressing b3a2 mRNA. CONCLUSION: bcr - abl Aspo has a specific growth inhibition effect on K562 cells, and worths further study in CML gene therapy.