ABSTRACT
Objective To establish the genetic and biochemical loci in Mesocricetus auratus and its albino mua-tant.Methods Protein isozyme cellulose acetate electrophoresis was used to determine the genetic and biochemical loci in Mesocricetus auratus and its albino mutant, using the genetic and biochemical loci of mice and rats.Results 25 biochemi-cal markers of Mesocricetus auratus and albino mutant were established, and polymorphism of their genetic biochemical loci was analyzed.Conclusions Polymorphism of biochemical loci is present in Mesocricetus auratus.Some differences exist between the genetic biochemical loci of Mesocricetus auratus and their albino mutant.These results laid the foundation for further study on genetic mechanism of albino mutation in Mesocricetus auratus.
ABSTRACT
In the present study, the effect of fibroblast-mediated IL-2 gene therapy on immune and hematopoietic reconstitution was observed after syngeneic bone marrow transplantation (BMT) in the mice which had received high-dose chemotherapy. The NK activity , LAK activity and the proliferation of BMT were augmented significantly , while there were no effects on the formation of CFU-GM, CFU-MK and CFU-E of bone marrow after IL-2 gene therapy. The results suggested that fibroblast-mediated IL-2 gene therapy can accelerate and prompt the process of immune reconstitution, then enchance the antitumor effect and reduce the complication such as infection after BMT. The experiment provide basis for the application of IL-2 gene therapy in BMT in the future.
ABSTRACT
By using 5'-nucleotidase-alkaline phosphatase (5'-Nase-AlPase) double staining method, the fine distribution of the lymph vessels in the stomach wall of the rabbit, guinea pig and rat has been observed. Under light microscope lymph capillaries showed strongly 5'-Nase positive reaction with brown or dark brown staining. However blood capillaries revealed significantly A1Pase activity with blue staining. The lymph capillaries were seen in all layers of the stomach wall in three species, but the lymph vessels were only shown in the submucosa, muscularis and serosa. In the mucosa lymph capillaries could be found in the deep layer of the lamina propria. In the submucosa there were lymph vessels and capillaries. In the muscularis lymph capillaries and vessels not only could be found between oblique and circular muscle or between circular and longitudinal muscle, but also among muscle fiber bundles of each layer. In the serosa there were larger lymph vessels situated near the muscularis. The observation on semithin and ultrathin sections also shown the similarity in the lymph vessels distribution. There was no difference in the distribution of lymph vessels in the stomach wall between rabbit, guinea pig and rat.