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ObjectiveTo explore the molecular mechanism of Shuyuwan regulating polarization of tumor-associated macrophages （TAMs） to inhibit the progression of colorectal cancer （CRC）. MethodThe nude mouse model of orthotopic transplantation of colon cancer was established. Male BALB/c-nu nude mice （n=28， 4 weeks old） were randomly assigned into 4 groups （n=7）： Model group （normal saline） and low-， medium-， and high-dose （1.725， 2.310， 2.895 g·kg-1·d-1， respectively） Shuyuwan groups. On day 9 after the tumor block was inoculated， the mice were administrated by gavage with corresponding agents at a dose of 15 mL·kg-1 once a day， 6 days a week， and no agent on the 7th day. After two consecutive weeks of intervention， the nude mice were sacrificed and the tumor samples were collected. A part of the colon tissue and the tumor tissue was used to prepare sections， and hematoxylin-eosin （HE） staining was performed for pathological observation. The expression of inducible nitric oxide synthase （iNOS） and arginase-1 （Arg-1） in the tumor tissue was detected by immunohistochemistry （IHC）. The mRNA levels of interleukin-12 （IL-12）， epidermal growth factor （EGF）， and transforming growth factor-β1 （TGF-β1） in the tumor tissue were determined by Real-time polymerase chain reaction （PCR）. Western blot was employed to determine the protein levels of iNOS， IL-12， EGF， and TGF-β1 in the tumor tissue. ResultCompared with the model group， Shuyuwan inhibited the growth of colon cancer cells in nude mice and caused the tumor cell necrosis in different degrees. The high-dose Shuyuwan group had the strongest inhibitory effect on the growth of tumor cells， which basically lost the normal morphology. Furthermore， Shuyuwan up-regulated the expression of iNOS and IL-12 in M1-type macrophages （P<0.05） and down-regulated the expression of Arg-1， EGF， and TGF-β1 in M2-type macrophages （P<0.05）， which indicated the weakened polarization of macrophages toward M2 type and the enhanced polarization toward M1 type after treatment with Shuyuwan. ConclusionShuyuwan can inhibit the growth of orthotopically transplanted colon tumor by blocking the polarization of TAMs to M2 type and promoting the polarization of TAMs to M1 type.
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Objective: To analyze the incidence, etiological factors, clinical characteristic, diagnosis, treatment method and the prevention measures of recurrent ectopic pregnancies in our hospital from January 2001 to June 2004. Methods:19 cases of recurrent ectopic pregnancy were analyzed retrospectively. Results: There were 19 recurrences in 353 cases of ectopic pregnancy with a incidence rate of 1∶ 18.6( 19/353). The average age was 31.7 years old. 17 were operated with lesions involving the contralateral oviduct in 16 cases and the homolateral oviduct in 1 case. Pathological examination revealed additional chronic salpingitis in 13 cases. The shortest interval between the recurrent ectopic pregnancies were 2 months to 6 years,with an average of 33 months. Among them, 10 patients recurred within 36 months. There was one patients suffering from 3 times of ectopic pregnancies. The misdiagnosis rate was 5.26%. And the disease of misdiagnosis was miscarriage of early pregnancy. Surgical treatment can be the first choice of treatment of recurrent ectopic pregnancy. Conclusion: Pelvic adhensions, inflamed salpinx and conservative treatment are risk factors of recurrent ectopic pregnancy. Effective treatment of pelvic inflammation and avoiding of the first heterotopic pregnancy are the important measures for prevention of recurrent ectopic pregnancy.
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Objective: To analyze the pathogenesis,diagnosis,treatment and prognosis of severe hypertensive disorder complicating pregnancy(HDCP) and hypoproteinaemia(HP).Methods: We reviewed 25 cases of HDCP and HP treated in our hospital and compared and analyzed the perinatal and maternal outcomes,including pregnancy edema,serum albumin,delivery methods and the weight of newborns.Results: Severe HP was the main cause of pregnancy complications in severe PIH.Prompt correction of HP benefited both the neonate and the mother.Conclusion: Severe HDCP is usually complicated with severe HP.Prenatal correction and treatment of HP will be helpful for both severe HDCP mothers and the newborns.
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<p><b>OBJECTIVE</b>Compare two methods of preparing human unfertilized oocytes interphase nucleus and analyze the relationship between the different in vitro fertilization(IVF) indications, ovarian stimulation protocols, women's age and frequency of chromosome 17 aneuploidy.</p><p><b>METHODS</b>Tarkowski's air-drying method(3:1 methanol:acetic acid) and Coonen's 0.1% Tween 20/0.01 mol/L HCl method were used to fix human unfertilized oocytes interphase nucleus, and telomeric probe of 17qter was used by standard fluorescence in situ hybridization(FISH) procedures to confirm chromosome 17qter aneuploidy.</p><p><b>RESULTS</b>Of 36 human unfertilized oocytes, 24 were haploid (66.7%), 7 were disomic (19.44%), 5 were trisomic (13.89%). The overall frequency of aneuploidy was 33.3%. There were no differences between the protocols characterized by different maternal age, IVF indication, ovarian stimulation.</p><p><b>CONCLUSION</b>Tarkowski's air dry method is as good as the method of Coonen's, but the latter method can avoid the smell pollution of the methanol and acetic acid, and it is easy to operate. The chromosome 17 aneuploidy is one of the factors to cause in vitro fertilization failure of human oocytes.</p>
Subject(s)
Adult , Female , Humans , Aneuploidy , Cell Nucleus , Genetics , Chromosomes, Human, Pair 17 , Genetics , DNA Probes , Diploidy , Embryo Transfer , Fertilization in Vitro , Methods , Haploidy , In Situ Hybridization, Fluorescence , Methods , Interphase , Genetics , Karyotyping , Microscopy, Fluorescence , Methods , Ovulation Induction , Methods , Ovum , Physiology , TrisomyABSTRACT
60,≤60 year age groups and control group was 2.49?1.82,1.82?1.58,0.90?1.38, significantly decreased(P