To study the relationship of caffeine elimination and Child's Pugh classification in groups of patients with liver cirrhosis

Serum Caffeine clearance determination is a useful method to evaluate the severity of liver disease and predict short-term survival of cirrhotic patients. Caffeine concentrations correlated well with the score indicating the sufficiency of the organ according to Child- Turcotte classification score. It offers another choice for the quantitative measurement of liver functional reservoir. Study is designed to find out the relationship of Caffeine elimination and child's classification in groups of patients with liver cirrhosis. Cross sectional Study. The study was conducted at the Medical Ward of Services Hospital Lahore from July 2004 to December 2004. Forty patients with liver cirrhosis with age range 40-55 years and 20 aged matched normal healthy volunteers were enrolled in this study. Patients were taken from the medical ward of Services Hospital Lahore. After an overnight fast, the first blood sample was collected at 8 a.m., immediately followed by an oral administration of 200 mg Caffeine. Subsequent samples of venous blood were obtained at 8.30 a.m., 9 a.m., 11 a.m., 2 p.m. and 5 p.m. The Caffeine clearance was determined by reversed-phase high pressure liquid chromatography using a Phenomenex Gemini C18 column using a wavelength of 273 nm. [Jaundice presented with highest frequency distribution and percentages. This was followed with ascities, edema. Family history of hepatitis/jaundice and anemia. Ultrasonographic findings showed that in 50% of the patients. Other 50% of patients have normal and shrunken liver]. Caffeine concentration in child class A was significantly decreased at base line [P<0.001], 3 hours [P<0.05], 6 hour [P<0.001] and 9 hour [P<0.001] as compared to the controls. In child class B the Caffeine concentration was significantly to the controls. On the other hand in child class C the Caffeine concentration was remaining same as in their controls. Caffeine clearance could provide a practical assessment of hepatic function in cirrhotic patients. Our data emphasize the value of the child-Turcotte or child-Pugh classification in assessing the severity of liver cirrhosis in a simple and less time-consuming way than using quantitative liver function tests

BMI and lipid profile: a serious health threat for middle aged women

Study tried to find out factors that associated with the weight gain or increased BMI in middle aged women and lipid profile that may become a cause of different disease in women in middle/later ages. Study tried to find out factors that associated with the weight gain in middle aged women and lipid profile that may become a cause of different disease in women in middle and also in later ages. Cross sectional Study. This study was conducted at the department of Biochemistry, Fatima Jinnah Medical College, Lahore from August 2010 to October 2010. 50 females with age range 40-50 years were included in the study. The proforma of questionnaire recorded the age, socioeconomic status, BMI, blood pressure, dietary pattern, Physical activity and family history of female subjects. Lipid profile included total lipid, serum cholesterol, triglyceride, HDL-chil and LDL-chol were also estimated. It was observed that mean age at which females gained the weight is 45.23 years. Most females belong to class B and Class C. Females were over weight when compared with the standard criteria of weight to height ratio. No change in blood pressure was seen. A positive family history of obesity was also seen in many females. It was observed that the level of total lipids, cholesterol, triglyceride and LDL-cholesterol was significantly increased in female subjects as compared to normal subjects. In view of this study, it is suggested that a diet is not simply a list of permitted food but it may include the total count of calorie a person consume in the form of food in addition to cut down some percent of sugar, salt and oil. A regular exercise may help to maintain the circulation of blood and maintain the level of lipid profile