ABSTRACT
Background and aim: Diabetes mellitus is considered as an important risk factor for clinical disorders such as nephropathy, neuropathy, retinopathy and cardiovascular diseases. Medicinal plants, due to low side effects, are considered as suitable alternativer chemical drugs. Therefore, the aim of this study was to assess the effect of artichoke [Cynara scolymus L.] aqueous extract on biochemical factors in Sterptozotocin-induced diabetic rats
Methods: In this experimental study, 24 male rats were divided into 4 groups. One group [control] received standard diet and the other three groups were injected by sterptozotocin to induce diabetes. Then one, among the three groups, received standard diet [diabetic control group] and the other two groups received 200 and 400 mg/kg artichoke aqueous extract for 21 days by stomach tube, respectively. At the end of the experiment, the blood samples were collected through cardiac puncture and serum triglyceride, total cholesterol [TC], glucose, HDL-C, LDL -C, VLDL-C, HbA1c, superoxide dismutase [SOD] activity of red blood cells [RBC], plasma malondialdehyde and antioxidant capacity were measured and compared by ANOVA and Tukey tests
Results: Artichoke extract significantly reduced serum TC, TG, VLDL-C, glucose, plasma malondialdehyde and HbA1c in treated diabetic rats as compared to diabetic control group [P<0.05]. Additionally, artichoke extract significantly increased the plasma antioxidant capacity, HDL-C, and superoxide dismutase activity of RBC in treated diabetic rats, compared to the diabetic group
Conclusion: The findings of this study showed positive effects of artichoke extract on lipoprotein profile, antioxidant status, and glucose tolerance in diabetic rats. Therefore, artichoke extract may be beneficial in control of diabetes, abnormalities in lipid profiles and reduction of oxidative stress by activation of SOD activity
ABSTRACT
Antibody secretion in human may be the result of the changes in protein structure, or polymorphism in human thyroid peroxidase [TPO] gene that are the reason for presence of the Anti TPO. In this study we examined the association between of A1936/G exon11 polymorphism of TPO gene in respect to Anti-TPO level and in a sample population of Tehran. We enrolled 190 individuals with 47 +/- 2 y an average age of case-control groups. PCR-ARMS was used to amplify the segment of exon11 polymorphisms. In exon 11, the genotype frequencies were in conformity with the Hardy-Weinberg expectation. The results of this study show that the frequencies were 0.7298 for the G allele and 0.2710 for A allele. The presence of the G allele is significantly associated with increased anti-TPO level [GG: 238 +/- 57 IU/ml vs. AA: 74 +/- 33 IU/ml, P<0.05]. The G allele frequenc in the group with anti-TPO>100, was higher than that of the A allele. The results indicated that G allele carriers of exon 11 are exposed to increased Anti-TPO levels higher than A carriers