[ laboratory study on human jaw osteoblastic stem cells culturing]

The goal of this study was to evaluate different methods of cultivating human bone cells. Five periosteal and bone specimens obtained from the human jaw were divided into small pieces in the laboratory. After the addition of trypsin and collagenase enzymes and releasing of cells, three primary periostal, endosteal, and bony chips cells were prepared. After passing the required time for the growth of specimens, lamella were prepared and stained with alkaline phosphatase [ALP] in order to determine ALP positive cells. Mean time of cellular growth was 23 days. Human bone cells have the capability of being cultured under special sterile laboratory conditions and three dimensional culturing of them can be used for reconstruction of maxillofacial region defects

[Comparison of isolated anterior cruciate ligament tearing with anterior cruciate ligament tearing associated with displaced lateral femoral condyle fracture in knee arthrosis formation]

The etiology of posttraumatic arthritis has not been studied well. Although it has long been assumed that articular incongruity leads to post traumatic arthritis, clinical and basic sciences evidence to support such claim are not sufficient. On the other hand, the relationship of instability due to anterior cruciate ligament deficiency and Arthrosis has not been clearly defined and the relative importance of instability and incongruity is unknown. ACL tearing were created in right knees of 9 rabbits [group A] and ACL tearing and displaced lateral condylar fracture with 1 mm step were created in another 9 rabbits [group B]. After 6 months, osteoarthritis changes were compared between control knee and injured knee of each rabbit and between the two groups. In both groups, osteoarthritis changes in injured knees were more comparing to the control knee and in whole osteoarthritis changes were significantly more in group B compared to group A. The unstable knee due to ACL deficiency goes toward osteoarthritis faster than normal knee. Incongruity in unstable joint increases intensity of osteoarthritis changes. It shows the importance of achieving anatomic reduction in intra articular fractures before ligament reconstruction