ABSTRACT
Braxy vaccine, a culture of a suitable strain or strains of Clostridium septicum in a fluid medium, its filtrate or derivatives, will be inactivated so that it produces its immunogenic activity without toxicity. Production of C. septicum vaccine [Braxy vaccine] using enriched culture media in fermenter. Conventional and enriched culture media were used for growing C. septicum [CN913] vaccinal strain in both 10 liter glass bottle and fermenter, in a triplicate manner. All twelve experiments were inspected to ensure compliance with the requirements of the vaccine international standards for minimum lethal dose [MLD], sterility, freedom of abnormal toxicity, safety and potency tests. Results showed that the culture of C. septicum in fermenter using enriched culture media is the best. Based on international standards, 2.5IU/mL was determined for C. septicum alpha toxin potency test, but in this study 4IU/mL was obtained. Using enriched culture media in fermenter is suitable for braxy vaccine production