ABSTRACT
Q fever is a zoonotic disease caused by Coxiella burnetii, a species of bacteria that is distributed worldwide. In cattle, Coxiella burnetii infections are generally asymptomatic but can also be associated with reproductive disorders. The aim of this study was to achieve molecular detection of Coxiella burnetii in dairy bovine milk farms using Nested PCR in Qom province, Iran. From January to February 2011 [winter] and July to September 2011[summer] a total of 100 bovine bulk milk samples were equally collected from five areas of Qom. The nested PCR assay used to screen for C. burnetii was designed from the nucleotide sequence of the com1 gene encodin a 27-kD outer membrane protein [OMP]. In this study, 14% [14 of 100] of bulk milk were positive. These results support the hypothesis of high prevalence and endemic pattern of Q fever in Qom province of Iran
ABSTRACT
Manganese related jobs [Mn miners] may cause Manganism, a Parkinson like disease. Chelators are suggested to reduce these effects. This study was carried out to investigate the effect of 4-Amino Salicylic acid chelating effects. This experimental study was done on 20 adult male Wistar rats. The rats were classified into four groups, 5 in each group, as follows: one control and three experimental groups. In two phases the groups received Salin-Salin, Manganese-Salin, Salin-4-Amino acid and Manganese -4-Amini acid, respectively. Eight mg/kg of manganese chloride i.p. injection for 1 week and 1.5g/l of PAS s.c injection five-day in a week for four weeks, were used for intoxication and treatment, respectively. Serum and liver tissues were separated. Data were analyzed using ANOVA and Tukey's tests. Biochemical factors showed cholesterol increment in group 2 compared with group 1 and decrement of cholesterol level in the blood of group 4 compare with group 2 [P<0.01]. Also increasing the direct billirubin in the Mn and PAS groups was more significant compared with control group [P<0.05]. Billirubin was observed in the urine of the Mn, PAS, Mn+PAS groups and urobilinogen declining was decreased in the urine of these groups [P<0.05]. There was a significant increase [P<0.05] of OT, PT enzymes in the serum of PAS and ALP in the Mn and Mn+PAS groups compared with control group. PAS can play a significant role in reducing destructive effects of Mn, especially on liver but has different side effects, like other chemical drugs
ABSTRACT
The aim of the present investigation was to compare the healing process of repaired Achilles tendons of healthy rats and streptozotocin induced diabetic [STZ-D] rats by evaluating strength and toughness of repaired tendons via tensiometrical test. 14 healthy male adult rat were divided into control [n=6] and experimental rats [n=8]. Type 1 diabetes was induced in experimental rats by an injection of 55 mg/kg STZ intraperitoneally. Control rats were received distilled water. Blood Sugar of all rats was recorded after seven days. Animals of experimental group that failed to develop average blood glucose concentration greater than 250mg/dc were excluded from the study. Right Achilles tendon of all rats was transected completely 30 days after STZ injection. Repairing Achilles tendons were extracted and were submitted to a tensiometerical examination10 days after surgery. Data were compared by student t test. Independent sample t test analysis showed that Young's modulus of elasticity [18.5 +/- 12.1MPa/mm] and stress high load [3.04 +/- 1.38, MPa/mm] of control group were significantly higher than those of experimental group [2.5 +/- 2 and 0.975 +/- 0.269 respectively], [p=0.003 and p=0.004 respectively]. It is concluded that induction of type one diabetes by STZ in rats after 30 days reduced significantly tensiometrical parameters of repairing Achilles tendon in comparison with control rats