Physio-chemical properties of glucose-oxidase

Physical and chemical properties of glucose oxidase from Penicillium chrysogenum were investigated. Results showed that the enzyme is highly specific for b-D-gluco-pyranose. Removal of CH2O group to form pentose reduced the rate of oxidation to zero. Different hexoses such as galactose, mannose and fructose were studied. Analytical results for the enzyme showed that it contained 17% followed residues. Mannose was the most abundant [12%] followed by glucosamine [3.4%] and galactose [1.5%]. Application of the enzyme preparation for glucose determination in different materials was studied