Induction toxoplasma gondii in goats

The prevalence of anti-Toxoplasma gondii antibodies was investigated in goats slaughtered for food in various areas of the Giza province. Egypt. Two hundred and thirty blood samples collected from the slaughtered goats were used for the serologic diagnosis of T. goadii infection by the modified agglutination test [MAT] with cut-off value 1:25. The antibodies to T. gondii good/i were found in 44.3% [102/230] of goat sera. Experimental infection of goats with the infective stages of both locally isolated and RH strains of T. gondii was carried out. Persistence of T. gondii tissue cysts and lesions were demonstrated in the different tissues and organs of the experimentally infected goats by the histo-pathological examination, This study is the 1st assay of toxoplasmosis in Egyptian goats by MAT and is the unique successful determination of the T. gondii infective stages within the experimentally infected goat tissues by the histo-pathological examination. Consequently the finding results obtained scope the public health significance of goat meat as source of human infection

Some methods of inactivation or killing of encysted metacercariae in Tilapia muscles

Encysted metacercariae in muscles of fresh water fishes [Tilapia sp.] may be hazardous to consumer or fish-eating mammals, so the inactivation of these parasites by different methods of control such as chilling, freezing and irradiation were studied. Results indicated that chilling of Tilapia muscles at 5° is not enough to kill the encysted metacercariae, but simple freezing at [-10]° for a period not less than three days or deep freezing at [-20] for at least two days are enough to kill all of these encysted metacercariae. Irradiation of Tilapia muscles with small doses gamma rays not less than 300 k.rad is enough to inactivate the metacercariae encysted in these fish muscles. Trematodes of family, Heterophyidae are more resistant to freezing and irradiation than those of family Cyathocotylidae

Studies on some fish-borne trematodes in Egypt

Prohemistomum vivax, Haplorchis pumilio, Procerovum calderoni, and Echinochasmus perfoliatus digenetic trematodes of fish eating mammals and man were recovered from experimentally infected dogs with muscles of the following fresh water fishes, Tilapia spp., Glorias lazera, Bagrus bayad, and Shilbe mystus. P.vivax isolated from dogs infested with the muscles of any of these fishes, while H.pumilio and E. perfoliatus recovered from dogs infested with muscles of Tilapia spp. or Bagrus bayad fishes, but P.calderoni recovered from dogs infested with muscles of Tilapia spp., Bagrus bayad, or Shilbe mystus muscles. In this study Tilapia sp. and Shilbe mystus fishes are consider as a new second intermediate host for P.calderoni, while Bagrus bayad is a new second intermediate host for E. perfoliatus