Novel pan-lineage VP1 specific degenerate primers for precise genetic characterization of serotype O foot and mouth disease virus circulating in India

Analysis of the VP1 gene sequence of the foot and mouth disease virus (FMDV) is critical to understanding viral evolution and disease epidemiology. A standard set of primers have been used for the detection and sequence analysis of the VP1 gene of FMDV directly from suspected clinical samples with limited success. The study validated VP1-specific degenerate primer-based reverse transcription polymerase chain reaction (RT-PCR) for the qualitative detection and sequencing of serotype O FMDV lineages circulating in India. The novel degenerate primer-based RT-PCR amplifying the VP1 gene can circumvent the genetic heterogeneity observed in viruses after cell culture adaptation and facilitate precise viral gene sequence analysis from clinical samples.

A comprehensive overview on the role of phytocompounds in human immunodeficiency virus treatment / 中西医结合学报

Acquired immune deficiency syndrome (AIDS) is a worldwide epidemic caused by human immunodeficiency virus (HIV) infection. Newer medicines for eliminating the viral reservoir and eradicating the virus are urgently needed. Attempts to locate relatively safe and non-toxic medications from natural resources are ongoing now. Natural-product-based antiviral candidates have been exploited to a limited extent. However, antiviral research is inadequate to counteract for the resistant patterns. Plant-derived bioactive compounds hold promise as powerful pharmacophore scaffolds, which have shown anti-HIV potential. This review focuses on a consideration of the virus, various possible HIV-controlling methods and the recent progress in alternative natural compounds with anti-HIV activity, with a particular emphasis on recent results from natural sources of anti-HIV agents. Please cite this article as: Mandhata CP, Sahoo CR, Padhy RN. A comprehensive overview on the role of phytocompounds in human immunodeficiency virus treatment. J Integr Med. 2023; 21(4):332-353.

Assessment of dehydroepiandrosterone sulphate (DHEAS) and cortisol levels in saliva and their correlation to cervical vertebrae maturation method in males and females at different growth stages: a clinical study

ABSTRACT Objective: The aim of this in-vivo study was to assess the salivary dehydroepiandrosterone sulphate (DHEAS) and cortisol levels, and their correlation to the Cervical Vertebrae Maturation method (CVM) in males and females at pre-pubertal, pubertal and post-pubertal growth stages. Methods: 48 patients (24 males, 24 females) who were to undergo routine orthodontic treatment were screened according to the inclusion and exclusion criteria. Then subjects were grouped according to CVM stages, using lateral cephalogram, in pre-pubertal, pubertal and post-pubertal groups. Unstimulated saliva from the selected subjects was collected. DHEAS and cortisol levels in the salivary samples were estimated by Enzyme-Linked Immunosorbent assay (ELISA). Then they were compared to Cervical Vertebrae Maturation Method stages. One-way ANOVA test followed by Tukey's post-hoc test was used to compare the salivary DHEAS and cortisol levels between different CVM stages in males and females. Independent Student t-test was used to compare the mean salivary DHEAS and cortisol levels between different males and females in each CVM stage. Result: There was a progressive increase in salivary DHEAS and cortisol concentration as skeletal maturation progressed from CVM stages 1 and 2, CVM stages 3 and 4, reaching the highest value at CVM stages 5 and 6. Their levels were higher in males than females. Conclusion: The salivary DHEAS and cortisol levels can be useful as a potential indicator of skeletal maturation, to aid in the assessment of pubertal status.

RESUMO Objetivo: O objetivo deste estudo in vivo foi avaliar os níveis salivares de sulfato de dehidroepiandrosterona (DHEAS) e de cortisol, e sua correlação com o método de maturação das vértebras cervicais (CVM) em homens e mulheres nas fases de crescimento pré-puberal, puberal e pós-puberal. Métodos: 48 pacientes (24 homens, 24 mulheres) que se submeteriam a tratamento ortodôntico de rotina foram selecionados de acordo com os critérios de inclusão e exclusão. Em seguida, usando telerradiografia lateral, os indivíduos foram agrupados de acordo com os estágios CVM, em grupos pré-puberal, puberal e pós-puberal. Foi feita coleta de saliva não estimulada nos indivíduos selecionados. Os níveis de DHEAS e cortisol nas amostras salivares foram avaliados pelo teste ELISA (Enzyme-Linked Immunosorbent assay). Em seguida, foram comparados aos estágios do método CVM. O teste ANOVA de uma via seguido pelo teste post-hoc de Tukey foi usado para comparar o DHEAS salivar e os níveis de cortisol entre os diferentes estágios de CVM em homens e mulheres. O teste t de Student independente foi usado para comparar a média de DHEAS salivar e os níveis de cortisol entre diferentes homens e mulheres em cada estágio de CVM. Resultados: Houve um aumento progressivo no DHEAS salivar e na concentração de cortisol à medida que a maturação esquelética progrediu dos estágios CVM 1 e 2, para os estágios CVM 3 e 4, atingindo o valor mais alto nos estágios CVM 5 e 6. Seus níveis foram maiores nos homens do que nas mulheres. Conclusões: Os níveis salivares de DHEAS e cortisol podem ser úteis como um potencial indicador de maturação esquelética, para auxiliar na avaliação do estado puberal.

External Auditory Canal Cholesteatoma: Clinical and Radiological Features

Abstract Introduction External auditory canal cholesteatoma (EACC) is often misdiagnosed. Objectives To outline the clinical presentation of EACC, and to describe its radiological findings on high-resolution computed tomography (HRCT) of the temporal bone. Methods The clinical records of all patients diagnosed with EACC from April 2017 to March 2020 in a tertiary care center were retrospectively reviewed. The clinical presentation, the findings on the HRCT of the temporal bone, and the treatment provided were analyzed. Results A total of 9 patients, 7 males and 2 females, with a mean age of 30 years, were diagnosed with primary EACC. Six patients presented with otorrhoea, three, with otalgia, three. with hearing loss, and one with facial palsy. Some patients had multiple symptoms. The most common findings on otomicroscopy were destruction of the posterior and inferior canal walls, with cholesteatoma and intact tympanic membrane (six patients). Two patients had aural polyp, and one had a narrow ear canal due to sagging of the posterior canal wall. On HRCT, all nine patients showed soft-tissue density in the external auditory canal with erosion of the canal wall. The disease extended to the mastoid in eight cases, and to the cavity of the middle ear in one. There were three cases of dehiscence of the facial canal. Dehiscence of the dural and sinus plates was observed in two cases each. Eight patients underwent mastoidectomy, and one underwent debridement with canalplasty. Conclusion Review of the clinical and radiological findings is essential to reduce the rate of misdiagnosis.

Prospective identification of potential factors influencing stem cell mobilization and the necessity for plerixafor use in newly diagnosed multiple myeloma patients undergoing autologous stem cell transplantation

ABSTRACT Introduction: To study the efficacy and safety of single large volume leukapheresis by using generic G-CSF or G-CSF plus Plerixafor in achieving adequate stem cell yield and various factors influencing thereof in newly diagnosed multiple myeloma patients undergoing autologous stem cell transplant . Method: This prospective study was undertaken among 55 newly diagnosed multiple myeloma patients undergoing autologous stem cell transplant and aged between 18 and 75 years. Mobilization and harvesting of stem cells were performed by using GCSF or GCSF plus Plerixafor and large volume leukapheresis, respectively. A stem cell yield of ≥2 × 106 kg-1 and the number of apheresis procedures were primary efficacy endpoints, while the ideal stem cells yield >5 × 106 kg-1, the engraftment day and D100 response/graft sustainability were secondary endpoints. Result: The primary endpoint was achieved in all cases in both the groups by using a single LVL leukapheresis procedure. Fulfillment of all the secondary endpoints was satisfactory and comparable in both the groups. Age, pre-apheresis CD34+ count and number of interruptions during the LVL were significant factors influencing the stem cell yield (p < 0.05). Adverse drug reactions during the apheresis and post-ASCT period were manageable. Conclusion: The LVL is safe and cost-effective in attaining a minimum of CD34+ cells in a single procedure with manageable adverse reactions. Judicious intervention during the procedure may be helpful in ensuring the adequate yield.

Monostotic fibrous dysplasia of temporal bone: a case series and literature review

Fibrous dysplasia is a benign, progressive fibro-osseus disease. Typically, there are threevariants monostotic, polyostotic and McCune Albright syndrome. Monostotic variant being the commonest of all, affects mainly the craniofacial bones and ribs. Fibrous dysplasia affecting the temporal bone may be challenging at times. Here, we present our case series of three temporal bone fibrous dysplasias along with the literature review.

A Study to Develop and Evaluate a Need-based Curriculum on Nutrition for Students of Madrasahs in a Rural Block of Hooghly District, West Bengal

Introduction: Children are the hope and future for a nation.But, a large section of children is effected from malnutritionis also due to lack of knowledge and practices regarding theirfood requirements. So this study was conducted to designa training module to conduct more focused and need-basededucational interventions.Material and Methods: The present study was anintervention pre-post study on healthy life style among 187Madrasahs students of class VII and VIII where interventiongiven to the study Madrasah with the help of need basedcurriculum through trained teachers where teachers weretrained beforehand by the researcher himself.Results: The mean pretest knowledge score in study Madrasahwas 8.79 ± 4.15 and post test score was 24.19 ± 3.19.Thecalculated t-value was 33.34 and P value was less than 0.05with effect size 4.160 (Cohen’s D). In control Madrasah themean pretest knowledge score was 9.11± 4.45 and post testscore was 10.78 ± 4.32. The calculated t-value was 1.82 and Pvalue was 0.07 with effect size 0.061 (Cohen’s D).Conclusion: This study was conducted to develop andevaluate a need-based training curriculum for madrasahstudents of rural West Bengal on nutritional knowledge andpractices. This training curriculum was implemented bytrained teachers who were. The finding of the study mayprovide adequate evidence to conduct more focused and needbased educational interventions to equip school students withage appropriate knowledge and practices.

Pregnancy outcomes of normal versus abnormal cardiotocography in a tertiary centre in Nepal

Background: Pregnancy and childbirth is normal physiological process with great pathological potential. The obstetricians are more concerned with the early recognition of fetal distress during labour and such an adverse outcome should be detected at the earliest point of time by an effective surveillance method. Cardiotocography as a part of biophysical profile has become an established diagnostic tool for fetal surveillance. To compare early perinatal outcome of normal and abnormal cardiotocography in terms of APGAR scores, need for neonatal resuscitation, NICU admission, perinatal death and mode of delivery.Methods: It was a cross-sectional study. 200 nulliparous/multiparous women with singleton pregnancy in cephalic presentation at gestational age 37-42 weeks in latent stage of labor were enrolled in the study and subjected to admission test in left lateral position using fetal monitor. Baseline FHR and contraction pattern were determined for 20 minutes and classification of patients was done into normal, and suspicious or abnormal according to the FIGO guidelines 2015.Results: Low APGAR scores, rate of LSCS, need for neonatal resuscitation, neonatal admission were more in the abnormal cardiotocography group. Cardiotocography in the current study has high sensitivity and high negative predictive value for detecting fetal distress.Conclusions: From the analysis of this study, it would be safe to conclude that an ominous cardiotocography should be managed appropriately without delay and obstetrician should be vigilant in suspicious as well as in normal admission test group for timely intervention for bettering the neonatal outcome.

A study to develop and evaluate a need-based curriculum on addiction for students of madrasahs in a rural block of Hooghly district, West Bengal

Background: Tobacco smoking habit is imbibed at a very early stage of life and once it is taken up it becomes very difficult to give up. In fact the vast majority of tobacco users worldwide begin the use of tobacco during adolescence. Objective of the study was to develop and evaluate a need-based training curriculum on healthy life style in terms of addiction for Madrasah students of rural West Bengal.Methods: It was a school-based health educational intervention study in rural area of Hooghly district, West Bengal among 189 Madrasahs students.Results: The mean pre-test knowledge score in study Madrasah was 6.06±2.69 and post test score was 10.83±1.69. The calculated t-value was 24.06 and p value was 0.00 with effect size 2.12 (Cohen’s D). The observed increase in the knowledge of consequences of smoking in the study Madrasah after the health education was statistically significant. There was no significant difference in the knowledge of the control Madrasah on the health consequences associated with cigarette smoking in pre and post-test.Conclusions: As adolescent period is the ideal period to impart life-style related training it is obvious that vital opportunities are being missed due to lack of need-based training on life-style issues for this important population group. So, a well-designed need-based health educational intervention may play active role in bringing desired knowledge and behavior among the population group.

Study of Anaerobic Bacteria in Dental Abscess in a Tertiary Care Hospital

Majority of anaerobes involved in dental infections are thought to be endogenous in origin. Due to breech of continuity of pulp chamber bacterial colonization occurs. Responsible pathogens are polymicrobial. If left untreated in early stages, it can act as foci of disseminated infections and spread rapidly to adjacent structures leading to life threatening conditions. Aims: The present study was undertaken to identify different anaerobic organisms and their association with risk factors. Methods: 40 pus samples were collected after mouth wash from patients presented with dental abscess. Samples were processed immediately for aerobic and anaerobic culture. After comparing with the aerobic culture, obligate anaerobes were checked for aero tolerance. Subculture done for identification of species by Gram stain, colony morphology and conventional biochemical tests. Final identification was done by Vitek 2 system. Results: 40 (100%) samples were culture positive. Total 60 bacterial isolates recovered from this 40 samples. Out of which aerobes 36 (60%) and anaerobes 24 (40%) isolated. Aerobes present in 18 (45%), anaerobes present in 12 (30%) cases and mixed aerobic and anaerobic flora in 10 (25 %) cases. Predominant isolates were anaerobic cocci, Peptostreptococcus micros (41.6%) followed by Peptostreptococcus anaerobios (25%).Diabetes mellitus, bad chewing habits, poor oral hygiene found as significant risk factors. Conclusion: This study highlights polymicrobial nature of infections and role of anaerobes play as pathogens. Early diagnosis and interventions are extremely important to prevent systemic complications. One should have a high index of suspicion of anaerobes while dealing with dental infections.

Morphine Consumption in a Tertiary Care Hospital in Eastern Nepal

Introduction: Oral morphine is extracted from a naturallyoccurring opium plant. It has been reviewed as a potentanalgesic and according to the World Health Organization(WHO) its use is recommended as the drug of choice for thetreatment of severe pain, including cancer pains and variousother types of pains. However, a large proportion of doctorsare hesitant to prescribe morphine because of the unfoundedfear of addiction. The main aim of this study was to assess themorphine prescribing pattern of doctors in conformity withthe international guidelines.Material and Methods: The present study was a descriptiveanalysis of oral morphine prescriptions made within a 6months period, between 2016 and 2017. All the prescriptionsfor the patients with pain under oral morphine in the hospitalrecords were reviewed.Results: The results showed that (53.6%) of all morphineprescriptions were from the Oncology Department/OPD,while the newly created Day Care Unit accounted for 43.9% ofthe prescriptions. Almost nil prescriptions were seen from theLabour ward. Only 1.2% of all the prescriptions conformed tointernational guidelineConclusion: The results reflected that there was a needfor more education and advocacy programmes to increaseawareness among doctors about morphine prescriptions.The cancer study revealed the useful information related toepidemiology of cancer among both the genders in Nepalwhich will prove to be useful in health planning and futureresearch.

A study on unhealthy cervix and its risk factors among currently married women of reproductive age group attending an urban health centre of Kolkata

Background: Cancer of cervix is the third most common cancer in women in India accounting for 21.7% of cancer deaths among women. Unhealthy cervix, characterized by presence of any abnormal growth, ulcer, or vasculature, is a clinically detectable early stage in the life history of cervical cancer. Very few studies have been conducted to determine risk factors of unhealthy cervix. Methods: A clinic-based cross-sectional study was conducted among currently married women of reproductive age group attending Urban Health Unit and Training Centre, Chetla, Kolkata. A total of 189 women attending the clinic was selected using systematic random sampling method was who were interviewed and examined using pre-designed pre-tested schedule. Analysis was done using (SPSS version 16). Results: Among 189 women, 45.5% were diagnosed with unhealthy cervix. Hierarchical logistic regression showed that educational status, PCI, frequency of cleaning external genitalia and extramarital relationship of the spouse were significantly associated with unhealthy cervical condition. Conclusions: Most of the risk factors of unhealthy cervix are behavioral and modifiable. Health education and behaviour change communication of women of reproductive age group would help in reducing their morbidity as well as mortality from cervix related conditions that includes cervical cancer.

Corrigendum to “Statistical Evaluation of Two Microbiological Diagnostic Methods of Pulmonary Tuberculosis After Implementation of a Directly Observed Treatment Short-course Program” Osong Public Health Res Perspect 2013;4(1):45–51

In the above-mentioned article, the authors want to update the source of Figure 1 both in Figure legend and in the Reference section, which was missing the original article.

Comparative Study of the Serum Bilirubin and Various Other Liver Related Enzymes in Different Types of Jaundice

Background: This study was designed to compare the study of serum bilirubin and various other Liver related enzymes in different types of Jaundice in patients attending the Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha. Methods: The study includes total 50 subjects, out of which 20 were controls and 30 were Jaundice cases. Thirty jaundice cases were divided into 10(Pre-Hepatic), 10(Hepatic) & 10(Post-Hepatic) jaundice patients according to inclusion-exclusion criteria. Results: The results of our study show that the levels of Total Bilirubin, Direct Bilirubin & Indirect bilirubin were significantly raised in the Jaundice patients than that of control. This study was found that the other liver enzyme i.e. SGOT, SGPT & ALP were found to be increased than the control. Conclusion: In conclusion, the high serum bilirubin level is a marker of Jaundice. Increased direct bilirubin and alkaline phosphatase (ALP) specifically points towards obstructive Jaundice, Increased indirect bilirubin level points towards pre-hepatic (Hemolytic) jaundice whereas increased bilirubin (Direct & Indirect) along with SGOT & SGPT levels points towards hepatic jaundice.

Bayesian analysis of high-resolution ultrasonography and guided fine needle aspiration cytology in diagnosis of palpable thyroid nodules / Análise bayesiana de ultrassonografia de alta resolução e citologia de aspiração poragulha fina guiada no diagnóstico de nódulos palpáveis da tireoide

Abstract Introduction To evaluate diagnostic accuracy of high-resolution ultrasonography in differentiation of benign and malignant thyroid nodules in comparison to results of guided fine needle aspiration cytology based on the Bayes rule. Objective To assess the validity of ultrasonography results of thyroid nodules in comparison to guided fine needle aspiration cytology findings. Methods This study was done on randomly chosen 80 patients presented with palpable thyroid nodules, undergone real-time sonographic evaluation of thyroid nodules to characterize features, internal consistency, margins, echotexture, calcification, peripheral lucent halo and vascularity. Ultrasonography guided fine needle aspiration cytology studies of thyroid nodules were done. Results Palpable thyroid nodules were highly prevalent in fourth and fifth decades of life with female-male ratio, 4:1. Solid internal consistency was demonstrated by 75% malignant nodules. Hypoechogenicity and intra-nodular micro-calcifications were observed in 92% malignant nodules; 83% malignant nodules had intra-nodular vascularity and absence of peripheral halo. The pre-test prevalence of malignant nodules in the targeted population was 17.5%. As type I error, 2.5% false-positive cases and as type II error, 5.0% false-negative cases were detected. Values of sensitivity and specificity of the ultrasonography test were 71.43 and 96.97%, respectively. Conclusion Malignant thyroid nodules demonstrated ultrasonography characteristics of hypoechoic texture, intra-nodular micro-calcifications, solid consistency, internal vascularity and absence of peripheral halo. The ultrasonography test has 92.5% diagnostic accuracy to differentiate malignant from benign lesions in comparison to the gold standard fine needle aspiration cytology test.

Resumo Introdução Avaliar a precisão diagnóstica da ultrassonografia de alta resolução na diferenciação de nódulos tireoidianos benignos e malignos em comparação com os resultados da citologia de aspiração por agulha fina baseada na regra de Bayes. Objetivo Avaliar a validade dos resultados da USG de nódulos da tireoide em comparação com os resultados obtidos por citologia de aspiração por agulha fina. Método Este estudo foi feito em 80 pacientes selecionados aleatoriamente, que apresentavam nódulos palpáveis da tireoide, submetidos à avaliação ultrassonográfica em tempo real de nódulos da tireoide para estabelecer características, consistência interna, margens, ecotextura, calcificação, halo lucente periférico e vascularização. Foram feitos estudos por citologia de aspiração por agulha fina guiados pela USG dos nódulos de tireoide. Resultados Nódulos palpáveis da tireoide foram altamente prevalentes na quarta e quinta décadas de vida com uma razão sexo feminino-masculino de 4:1. A consistência interna sólida foi demonstrada em 75% de nódulos malignos. Hipoecogenicidade e microcalcificações intranodulares foram observadas em 92% de nódulos malignos; 83% dos nódulos malignos apresentaram vascularidade intranodular e ausência de halo periférico. A prevalência pré-teste de nódulos malignos na população alvo foi de 17,5%. Como erro tipo I, houve 2,5% de casos falso-positivos e como erro tipo II, foram detectados 5,0% de casos falso-negativos. Os valores de sensibilidade e especificidade do exame por USG foram de 71,43 e 96,97%, respectivamente. Conclusão Nódulos malignos da tireoide mostraram características de textura hipoecoica, microcalcificações intranodulares, consistência sólida, vascularidade interna e ausência de halo periférico na USG. O exame por USG tem 92,5% de precisão diagnóstica para diferenciar lesões malignas de benignas em comparação com o padrão ouro da citologia de aspiração por agulha fina.

Surveillance of bacteria Pseudomonas aeruginosa and MRSA associated with chronic suppurative otitis media / Vigilância de bactérias Pseudomonas aeruginosa e MRSA associadas à otite média crônica supurativa

Abstract Introduction: Suppurative otitis media is a critical disease causing perforation of the tympanic membrane associated with changes of the mucoperiosteum of the middle ear cleft. Objective: To isolate causative bacteria from chronic suppurative ear discharges and to ascertain their antibiotic profiles, of patients attending outpatients department in 3 years. Methods: For isolation of bacteria, samples of ear discharges were grown in suitable media and bacteria were subjected to antibiotic profiling by the Kirby-Bauer's method with presently used antibiotics. Results: A total of 1043 bacteria were isolated, including Pseudomonas aeruginosa and methicillin resistant Staphylococcus aureus, along with 121 fungal isolates. Among 371 P. aeruginosa isolates, tobramycin 30 had the highest susceptibility rate 93.2%, followed by ceftazidime 30, 91.5% and amikacin 10 µg/disk 64.4%. Of 359 S. aureus isolates, there were 236 coagulase negative S. aureus + methicillin sensitive S. aureus isolates, while 123 isolates were methicillin resistant Staphylococcus aureus with 95.2% isolates susceptible to cloxacillin 15, 83.3% isolates to erythromycin 15 and 78.5% isolates to gentamicin 30 µg/disk. Of 1164, 49 patients presented post aural abscess, 12 patients had intracranial complications, 9 patients had facial palsy and 3 patients had labyrinthitis. More than 90% P. aeruginosa and 90% S. aureus isolates were sensitive to tobramycin 30 and cloxacillin 30 µg/disk, respectively. Conclusion: Multidrug resistant strains of P. aeruginosa were more prevalent than those of S. aureus in ear discharges. Tobramycin and cloxacillin may be included in the formulatory antibiotic regimen to overcome bacterial infections in chronic suppurative otitis media.

Resumo Introdução: Otite média supurativa é uma doença importante que causa perfuração da membrana timpânica, além de alterações do mucoperiósteo da orelha média. Objetivo: Isolar as bactérias causadoras a partir da secreção auricular crônica e verificar seus perfis de sensibilidade aos antibióticos em pacientes ambulatoriais durante três anos. Método: Para o isolamento das bactérias, as amostras de secreções auriculares foram cultivadas em meios adequados e as bactérias foram submetidas à detecção de perfis de sensibilidade aos antibióticos com o método de Kirby-Bauer para antibióticos usados na atualidade. Resultados: No total, 1.043 bactérias, incluindo Pseudomonas aeruginosa resistente à meticilina e Staphylococcus aureus, e 121 fungos isolados foram identificados. Entre 371 isolados de P. aeruginosa, tobramicina 30 µg/disco apresentou a maior taxa de suscetibilidade (93,2%), seguida por ceftazidima 30 µg/disco (91,5%) e amicacina 10 µg/disco (64,4%). De 359 isolados de S. aureus, 236 eram S. aureus coagulase-negativos + S. aureus sensíveis à meticilina (MSSA), enquanto 123 eram MRSA com 95,2% de suscetibilidade à cloxacilina 15 µg/disco, 83,3% sensíveis à eritromicina 15 µg/disco e 78,5% à gentamicina 30 µg/disco. Entre 1.164 pacientes, 49 apresentaram abscesso aural, 12 complicações intracranianas, nove paralisia facial e três labirintite. Mais de 90% das P. aeruginosa isoladas e de 90% de S. aureus eram sensíveis à tobramicina 30 µg/disco e cloxacilina 30 µg/disco, respectivamente. Conclusão: Cepas multirresistentes de P. aeruginosa foram mais prevalentes do que as de S. aureus nas secreções auriculares. Tobramicina e cloxacilina podem ser consideradas na formulação de regime de antibióticos para tratar as infecções bacterianas na OMCS.

Validación estructural del wong-law emotional intelligence scale (wleis): estudio preliminar en adultos / Structural validation of the wong-law emotional intelligence scale (wleis): preliminary study in adults

La medición de la inteligencia emocional ha tenido muchas propuestas en formato de autorreporte. Una de estas es el Wong-Law Emotional Intelligence Scale (WLEIS), creada para el contexto laboral y que evalúa valoración y expresión de las emociones propias, valoración y reconocimiento de las emociones en otros, regulación de las propias emociones y uso de la emoción para facilitar el desempeño. Hay poca evidencia psicométrica sobre el Wong-Law Emotional Intelligence Scale en habla hispana, lo que posiblemente conduce a que sea también poco utilizado en la investigación y práctica profesional. El objetivo del presente estudio es presentar los primeros resultados de la validez de la estructura interna del WLEIS en adultos peruanos. Fueron 120 participantes (72 mujeres) entre 17 y 59 años, quienes respondieron al cuestionario mediados por internet. Se analizó la estructura interna mediante metodología de ecuaciones estructurales. Se halló una satisfactoria estructura de 4 factores y elevadas cargas factoriales de los ítems; las correlaciones interfactores fueron altas o moderadamente altas; y la consistencia interna fue buena. Se concluye que los primeros resultados son aceptables para asumir que el modelo de Wong-Law también es aparentemente generalizable.

The measurement of emotional intelligence has had many proposals in the form of self-report. One of these is the Wong-Law Emotional Intelligence Scale (WLEIS), created for the job context and which evaluates valuation and the expression of self-emotions; valuation and recognition of emotions in others; regulation of oneÆs own emotions and the use of emotion to facilitate performance. There is little psychometric evidence regarding the Wong-Law Emotional Intelligence Scale in the Spanish speaking world, which could lead to it also being little used in research and professional practice. The objective of this study is to present the first results of the internal structure validity of the WLEIS in Peruvian adults. There were 120 participants (72 women) between 17 and 59 years old who responded to the questionnaire mediated by the internet. The internal structure was analyzed using structural equations methodology. A satisfactory structure of 4 factors and high factorial loads of items was found. The inter-factorial correlations were high or moderately high û and the internal consistency was good. It is concluded that the first results are acceptable û to assume that the Wong-Law model is also apparently generalizable.

Non-Albicans Candiduria: An Emerging Threat.

Objective: The incidence of Candida has been on rise worldwide. Clinicians face dilemma in differentiating colonization from true candiduria. The species identification of Candida is important, as non albicans Candida species are increasing in number and more resistant to antifungal drugs. Material and methods: The present study was conducted at a tertiary care teaching hospital of North India with an aim of investigating prevalence of NAC spp. among Candida isolates from urinary tract specimens. Results: A total of 7627 urine samples were analysed in a tertiary care hospital. The Candida isolates (180) were further speciated by Gram stain, culture on sabouraud’s dextrose agar, germ tube test, sugar fermentation test. A total of 180 (2.36%) Candida species were isolated from 7627 urine samples. Among them non albicans Candida species were predominant (66.7%), compared to Candida albicans(33.3%).The rate of isolates of Candida species were more in females, 101 (56.1%) than in males 79 (43.9%). The highest isolation rates of Candida among uropathogens were found in age group above 60 years.The emergence of non-albicans Candida similar to the trends in the western countries should be a cause of concern in our country. Conclusions: NAC spp. have emerged as an important cause of urinary tract infections. Its isolation from clinical specimens can no longer be ignored as nonpathogenic isolate nor can it be dismissed as a contaminant. Proper surveillance of these fungal pathogens is important to improve quality of care in tertiary care setting.