ABSTRACT
Background: Streptomyces levis is a member of Actinomycetes family. Chemical compounds extracted from this bacterium have been used as antibiotics, anti-tumoral, anti-bacterial, anti-fungal, antiviral, anticancer factors. The purpose of this study was to investigate different metabolites of Streptomyces strains isolated from north west soils, of Iran and antimicrobial effects of them
Methods: The soil samples were taken from north west of Iran in 2010. The isolated bacteria were purified and identified by conventional methods. The metabolites of this bacterium were extracted by 7 different solvents [diethyl ether, dichloromethane, hexane, ethyl acetate, chloroform, methanol and water]. The antimicrobial effects of metabolites were tested by disk agar diffusion method on Gram-positive and Gram- negative bacteria. The metabolites with antibacterial effects were analyzed by gas chromatography-mass spectrometry [GC-MS]
Results: Metabolites produced from diethyl ether solvent affected Gram- positive and Gram- negative strains and 15 different compounds identified in the active metabolite of the bacterium. The main ingredients were: Docosanoic acid, methyl ester [11.578%], 9-12-octadecadienoic acid [2.961%], 5-Tetracosenois acid, methyl ester [8.389%], Bis [2-ethylhexyl] phthalate [2.153%], and D-alpha-Tocopherol [0.959%]
Conclusion: Results of this study showed that docosanoic acid, methyl ester [11.578%] and 5-tetracosenois acid methyl ester [8.389%], were the main metabolites of Streptomyces levis isolated from north west soils of Iran. Antimicrobial effects of extracts could be due to the presence of 1, 2-benzene dicarboxylic acid diisooctyl ester compounds and bis [2-ethylhexyl] phthalate in the metabolite