ABSTRACT
The aim of the study was to investigate the invivo antioxidant activity of ethanolic extract Premna corymbosa (Rottl.) root against streptozotocin induced oxidative stress in different organs (liver, kidney, brain, heart and pancreas) of rats. Ethanolic extract of P.corymbosa (Rottl.) root was administered orally (200 mg/kg body weight) and the effect of extract on enzymatic antioxidants like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and polyphenol oxidase (PPO), non enzymatic like vitamin C, vitamin E and glutathione. Lipid peroxidation like basal, ascorbate and peroxide induced lipid peroxidation were also estimated. Glibenclamide was used as standard reference drug. A significant increase in the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-Stransferase and reduced glutathione were observed in different organs of diabetic rats on treatment with 200 mg/kg body weight of P.corymbosa (Rottl.) root extract and glibenclamide for 30 days treatment. Both the treated groups showed significant decrease in lipid peroxidation, suggesting its role in protection against lipid peroxidation induced membrane damage. P.corymbosa (Rottl.) possesses antioxidant potential which may be used for therapeutic purposes mainly in the prevention of oxidative damage that occur during diabetes.