ABSTRACT
In this present work two colorimetric methods were developed based on donor-acceptor complexes of alverine citrate (ALV) and tapentadol (TAP) with cobalt thiocyanate. These methods were developed on Perkin Elmer LAMBDA 25 UV–VIS spectrophotometer with 1cm quartz cells. The colored species formed are the coordination complexes of the drugs (electron donor) and the central metal atom of cobalt thiocyanate (electron acceptor) which is extractable into nitrobenzene from aqueous solution. The reaction conditions were optimized and validated to achieve maximum colour intensity. The colored complexes show maximum absorbance measured at 625 nm for both ALV and TAP. The absorbances were found to increase linearly with an increase in concentration which was corroborated by the calculated regression coefficients (0.9998-0.9999). Linearity was obeyed in the range of 100-600 μg/ml for both ALV and TAP, respectively. The molar absorptivity, sandell’s sensitivity, LOD, LOQ and other validation parameters have been evaluated extensively as per ICH guidelines and all the parameters were found within the acceptance criteria for both methods. The proposed methods were proven to be more accurate, simple, precise and rapid by statistical validation of recovery studies and could be suitable for regular analysis.
ABSTRACT
This report describes analysis of paclitaxel, which is an antineoplastic drug used in the treatment of kaposi's sarcoma and cancer of the lung by isocratic high performace liquid chromatography with UV detection in pure form and rat plasma. The analysis was carried out using phenomenex C18 (250 x 4.6 mm, 5 μm particle size) column with a mobile phase consisting of acetonitrile and phosphate buffer (pH 5) in the ratio of 80:20%, v/v. Paclitaxel was eluted at the retention time of 5.3 min when operated at the flow rate of 1 ml/min and monitored by UV at 227 nm. Paclitaxel was extracted from rat plasma by simple LLE method using non- toxic ethyl acetate as extraction solvent. The linearity was accessed in the concentration range of 100-600 μg/mL with correlation coefficient of 0.9999 and percentage recovery of 99.86. The liquid chromatography method was extensively validated for linearity, accuracy, precision, LOD, LOQ and robustness. All these analytical validation parameters were observed to be satisfactory, which indicates the usefulness of method for determination of paclitaxel in pure form and rat plasma. No interfering peaks were observed during the analysis.