ABSTRACT
Matrix metalloproteinases [MMPs], the key enzymes responsible for matrix degradation, are derived from polymorphonuclear leukocytes during the early stages of periodontitis. The aim of this study was planned to determine the levels of GCF [gingival crevicular fluid] matrix metalloproteinase-8 [MMP-8] and metalloproteinase-9 [MMP-9] patients with periodontitis and in healthy controls. Levels of crevicular MMP-8 and-9 were determined by ELISA in subjects with healthy without any periodontal disease [n=10] and periodontitis [n=10]. Significantly higher crevicular MMP-8 and-9 were observed in cases of periodontitis compared to healthy adults. Crevicular MMP-8 and-9 may serve as biomarkers of periodontal disease and aid in early detection of periodontitis
Subject(s)
Humans , Adolescent , Adult , Middle Aged , Matrix Metalloproteinase 8/analysis , Matrix Metalloproteinase 9 , Periodontitis/diagnosis , Early Diagnosis , /analysisABSTRACT
To determine the association between levels of a marker of oxidative stress, 8- hydroxydeoxyguanosine [8-OHdG], in saliva and presence of periodontitis in smokers and non-smokers. Forty subject samples, as healthy control [n=16], periodontitis in smokers [n=11] and periodontitis in non-smokers [n=13] were recruited for the study. The 8-OHdG were analyzed in the saliva of smoking and non-smoking patients of periodontitis and healthy control. Salivary levels of 8-OHdG were significantly higher in subject with periodontitis who smoked as compared to non-smoker [6.82 + 0.18 ng/ml and 4.83 + 0.16 ng/ml]. Thus, measurement of 8-OHdG may prove to be useful in identifying periodontal disease and risk of tooth loss