[ antimicrobial effect of zatariamultifloraboiss essential oil against E.coli O157: H7 in minced beef during refrigerated storage as a replacement for chemical preservatives in order to maintain the consumers health]

Today, the consumers have more knowledge about the unwanted effects of synthetic antimicrobials in foods and they want food with more natural ingredients. There are many researches about antimicrobial effects of essential oils, but we did not found researches about Zatariamultiflora Bios on E.coli O157: H7 in minced beef. The aim of this study was to determinre the effect of Zatariamultiflora Bios essential oil on E.coli O157: H7 in minced. The antimicrobial effect of different concentrations of Zatariamultiflora Boiss essential oil at supplementation levels of [0, 0.005, 0.015, 0.03%] on E. coli O157: H7 was examined in minced beef. All of the above concentrations showed acceptable organoleptic properties in minced beef. Eo at 0.03% possessed a strong antibacterial activity against E.coli O157: H7 in minced beef. The correlation coefficient of different concentrations of Zatariamultiflora Boiss essential oil with logarithm of the numbers of E. coli O157: H7 was -0.701, -0.599 at 4 and 10°C respectively. It was found that effect of different concentrations of essential oil on growth rate of E. coli O157: H7 was statistically significant [p<0.01]. In this study, it was found that treatment of minced beef with different concentrations of essential oils showed an inhibitory activity against E. coli O157: H7 during storage at 4°C, but not at 10°C [p<0.01]. According to present study, storage at a proper refrigerated storage like 4°C is suggested. Zatariamultiflora Bioss essential oil can use as a natural preservative instead of chemical preservatives for meat products

Molecular characterization of a Salmonella typhimurium isolate from Caspian pony

Typhoid disease or salmonellosis is a common sickness in horses. In several epidemiological studies in hospitalized horses, several serotypes of Salmonella often are predominant in nosocomial infections. Transportation, overcrowding, dehydration, oral antimicrobial therapy and infections are the risk factors which may activate latent or subclinical salmonellosis. In this study, the occurrence of typhoid due to Salmonella serogroup B was considered in a Caspian ponies flock kept in a husbandry center of ponies around Tehran. During transportation of 19 ponies, two pregnant ponies aborted and four cases died because of acute septicemia. Pathological and bacteriological follow up showed salmonellosis. A multiplex poly-merase chain reaction [m-PCR] assay was used for detection and identification of Salmonella to confirm pathological and bacteriological studies. Salmonella typhimurium was isolated from bone marrow, mesenteric lymph nodes, liver and intestinal contents of died pony. Salmonella was not isolated from stools of other ponies. Pulsed Field Gel Electrophoresis [PFGE] and antibiotic susceptibility test were also performed. PFGE pattern was similar to the other collected isolates which have existed since more than 30 years ago in Iran. Because of importance of salmonellosis in ponies, using rapid methods are recommended to confirm the presence of Salmonella. Results showed that m-PCR permit to evaluate samples more rapidly than other methods and also can detect multiple genes simultaneously like virulence factors which declare virulence of the isolates and have surveillance significances

Presentation of dermatophyte lesions on different sites of adult cattle

Seventy cattle suspected to dermatophytosis were examined between March and April 2003. The samples were taken from different lesions by scraping method. Direct microscopic examination and culture were carried out. The mean of infection duration was 2 weeks. Forty-three cases were appeared with positive clinical signs of dermatophytosis and 27 cases were asymptomatic. The etiologic agent of the infection was only Trichophyton verrucosum. A significant relation was observed between the frequency of head and neck lesions and other sites. There was a significant difference between the frequency of head lesions and other sites. Since human dermatophyte infections due to Trichophyton verrucosum have been reported repeatedly in Iran, it is necessary to design a preventive program such as developing an effective vaccine against dermatophytosis into the future