ABSTRACT
Levofloxacin is one of the Fluroquinoline antibiotic groups, which affect on controlling infections, especially in reproductive organs. It has therapeutic use in numerous countries, but little information exists on the effects of Levofloxacin on spermatogenesis when it is used for infectious treatment. The current study was designed to determine whether Levo- floxacin influences testis tissue and spermatogenesis in rats
In this survey 50 male Wistar rats 6-8 weeks [250 +/- 10 g] were used: normal salin as sham and control groups and 3 treatment groups [0.03, 0.06 and 0.08 mg Levofloxacin/kg body weight] during 60 days. The experimental groups were daily gavages. After 60 days, they were anesthetized with ether and testes were taken for histopathology studies, sperm parameters evaluation and several hormone concentrations
Although testosterone concentration was not affected by Levofloxacin levels, follicle stimulating hormone [FSH] and luteinizing hormone [LH] concentration significantly increased by Levofloxacin consumption in 0.03 and 0.06 mg Levofloxacin/kg body weight groups [P<0.01]. Moreover, sperm concentration decreased linearly as Levofloxacin was increased [200, 192, 170, 128 and 75×106 sperm for control, sham, 0.03, 0.06 and 0.08 mg Levofloxacin/kg body weight, respectively, P<0.05]. Testis tissue cuts in experimental group when the amount dosage of Levofloxacin increased cells solidarity to the primary and secondary spermatogonia. Adding Levofloxacin linearly reduced spermatocyte cells and amount of all cells in semenifer pipes tube [P<0.05]
Levofloxacin as an antibiotic has histopathology effects on the spermatocyte cells, especially in high dose. Therefore, it might reduce fertility in male that requires further studies
Subject(s)
Animals, Laboratory , Testis , Spermatogenesis , Rats, Wistar , LevofloxacinABSTRACT
Burn wound, as a chemical and physical phenomenon, is a leading cause of mortality in the world. The aim of this study was to investigate the effect of Cinnamon zeylanicum extract on burn wounds in diabetic and non-diabetic male rats by local usage and injection method. In this experimental study, 48 Wistar male rats were divided into 2 groups of non-diabetic burn wound group and diabetic group which was divided itself into 4 subgroups. To create wounds, a hot iron seal placed on the skin. Testing duration was 21 days. At the end of every week, some photographs and measurement were taken. In diabetic control and local treatment group, a significant reduction was found on 14[th] day in wound surface in comparison of diabetic group that received extract. For local treatment, a significant reduction was observed in healthy treatment group on 7[th], 14[th] and 21[th] days. Both healthy treatment and control group who received injection had a significant reduction on 14[th] and 7[th] days compared to healthy control group. Application of cinnamon zeylanicum extracts would contribute to burn wound improvement and it seems more effective in non-diabetic group compared to diabetic group, and also the injection method was more effective. Perhaps, this is due to better absorption of Eugenol and Cinnamaldehyde in extract by injection rather than local treatment
ABSTRACT
Nowadays the medicinal herbs are considerably used to decrease the symptoms of drug withdrawal syndrome and facilitate this process. According to anti-pain and anti-anxiety effects of Hypericum scabrum, this research was done to study acute effects of hydroalcoholic extract of Hypericum scabrum on morphine withdrawal symptoms in adult male NMRI mice strain. In this experimental study, adult male mice were divided into two main groups of control and experimental. The control group received morphine and naloxone. The experimental group received morphine and 10, 20 and 30 mg/kg doses of Hypericum scabrum. During three days and three times of each day with five hours intervals, the animal received 10, 20 and 30 mg/kg doses respectively and in the fourth day they received 40 mg/kg drug subcutaneously [Marshall method]. In chronic method, 10, 20 and 30 mg/kg doses of Hypericum were injected to three groups of morphine intraperitoneally. To induce withdrawal syndrome in all groups, three hours after the last morphine injection, one dose of 5mg/kg of naloxone was injected intraperitoneally. In all groups, after naloxone injection the symptoms of morphine withdrawal including jumping, standing on two feet, climbing up the wall and weight loss was considered. Acute injection of the Hypericum scabrum could significantly decrease the symptoms of morphine withdrawal in comparison with morphine group. Decreasing symptoms of morphine withdrawal probably ordinates from Hypericum scabrum opioid-like effects
ABSTRACT
Diabetes mellitus [DM] is a disorder of insulin secretion and characterized by increased blood sugar and is diagnosed by the presence of hyperglycemia. Administration of insulin and some other hypoglycemic agents are base of the treatment of diabetes, which due to side-effects of insulin, more attention has been given to herbal medicine. This study was carried out with the purpose of determining the effect of marshmallow root on decrease of blood sugar in diabetic rats. In this study, 36 mature rats with mean weight of 180-250g were divided into six 6-rat groups. Diabetes was induced by intraperitoneal injection of 40mg/kg.bw streptozotocin to the animals. The extract was injected intraperitoneally to the rats at a dose of 200mg/kg of during 6 weeks. Also, the effect of glibenclamide was compared with that of marshmallow root for 40 days. Data were analyzed by mixed one-way ANOVA [repeated measurements in independent groups]. In this investigation, blood glucose level in the group treated with the extract had no significant difference as compared to the group treated with glibanclamide [at a dose of 0.5mg/kg]. According to the results of this study, hydroalcoholic extract of Althea root has hypoglycemic effects on the diabetized rats, which this effect is exerted by important compounds exist in Althea root, such as pectin, antocianine, cyanidine, and dioxybenzoic acid