ABSTRACT
Background: In COPD, acute exacerbation is the common problem during natural course. Studies of sputum samples using standard culture and molecular techniques have demonstrated that it is associated with increased prevalence of bacteria. Methods: 200 clinically diagnosed cases of AECOPD of age ≥45 years were recruited. Two sputum samples each were processed by conventional methods. Preparation of media, reagents, Gram staining, identification of culture isolates, different tests, including antibiotic sensitivity tests were carried out following standard laboratory. Results: The prevalence of AECOPD was more common in the age group of fifty six to sixty five years (43%) with ratio between male and female of 2.12:1. Klebsiella pneumonia was the predominant organism isolated in 42.55%, followed by Staphylococcus aureus in 28.73%, P. aeruginosa in 14.89%, E coli in 8.51%, CONS in 4.26% and S. pneumoniae in 1.06%. Gram negative bacteria were most sensitive to meropenem, imepenem, amikacin, followed by cefotaxime ceftriaxone, levofloxacin, Cefepime and aztreonam. Gram-positive bacterial isolates were most sensitive to linezolid (34.04%) followed by vancomycin (32.98%), cefoxitin (31.91%).Conclusion: Sputum culture is a good and simple diagnostic tool to study the etiology due to bacteria in AECOPD. Antibiogram helps in the formation of the correct treatment protocol, screening resistant pathogens and better drug for treatment, thereby helping to decrease the mortality and morbidity.
ABSTRACT
Rhizoctonia solani is a most widely recognized strong saprophyte with a great diversity of host plants. It is a first ever case of extensive human mycosis caused by Rhizoctonia solani in a 65-year-old diabetic and hypertensive farmer, with a history of head injury caused by fall of mud wall. Necrotic material collected revealed septate fungal hyphae with bacterial co-infection. Fungal culture on SDA at 25°C showed cotton wooly growth progressing to greyish-white to shiny metallic black colonies and identified on basis of septate mycelial growth without conidia, right angle branching, presence of compact hyphal forms and anastomosis between branching hyphae on LPCB mount.
ABSTRACT
Magic Bullet comprises of a specific synthesized drug in which metal ions play an important role. These drugs can be designed to treat and cure a specific disease by attacking on specifically diseased cells leaving aside the healthy ones, there by, increasing the efficiency of a drug. As metal ions are used since ages as immuno-modulators, they trigger the immunity, if combined with the durg. Thus, combination of drug can be chelated with metal ions, which can help to develop innovative strategies for preparing combinatorial drug, also known as “Magic Bullet” or metal complexes or mixed ligand complex in the light of the chelate hypothesis.
Subject(s)
Immunologic Factors , Chelation TherapyABSTRACT
A total of 44 strains of Vibrio 0139 serotype isolated between April and August 1993 at Sevagram (Wardha) were examined for expression of a number of biochemical and physiological characteristics. All strains fermented lactose within 24 h and belonged to Heiberg group III. Salt tolerance to 8 per cent NaCl was seen in 22.72 per cent strains. Haemolysis of sheep RBCs and haemagglutination of human 'O', chicken and rabbit RBCs was consistently positive. All the strains were sensitive to tetracycline and resistant to polymyxin B and cotrimoxazole.