ABSTRACT
Objective To establish an HPLC method for the fingerprint analysis of Spatholobus suberectus, so as to provide evidence for the quality control of it. Methods Separation was performed on a ZORBAX SB-C18 column (150 mm × 4.6 mm, 5 μm) and the mobile phase was acetonitrile-0.2% phosphoric acid with gradient elution. The flow rate was 1 m/min and the wavelength was 260 nm. The column temperature was 30 ℃. The cluster analysis (CA) and principal component analysis (PCA) were applied to studying the HPLC fingerprint and chemical pattern recognition. Results The HPLC fingerprint of S. suberectus has been established with 35 common peaks, and six of common peaks were identified. The values of similarity evaluation mostly agreed with the result of CA and PCA. Conclusion The application of HPLC combined with CA and PCA could quickly and objectively evaluate the quality of S. suberectus from different regions.