ABSTRACT
A wild lactic culture isolated from dahi (fermented milk) sample and characterised as L. casei D-34 was found to be significantly cytotoxic (34-36%) against three tumour cell lines, HeLa, HEp-2 and HFS-9. The cytotoxic substance (CS) was found to be in the culture supernatants, protein in nature, with a molecular weight ranging from 17,000-20,000. The crude culture supernatant was partially purified by dialysis and ion exchange chromatography as anionic, cationic and neutral fractions. Among the fractions, except for the anionic fraction, others were found to be highly cytotoxic against all three tumour cell lines. The cationic, neutral and pooled (anionic:cationic:neutral in 1:1:1 ratio) fractions showed 50, 70, 70% cytotoxicity against Hep-2 cells, 70, 88, 94% against HFS-9 cells and 50, 89, 90% against HeLa cells respectively. Pooled fraction was found to exhibit higher percent of cytotoxicity compared to individual fractions indicating a synergistic effect. (3H)-thymidine incorporation studies revealed that CS and its fractions inhibited DNA synthesis in tumour cells. The CS was stable towards heat and pH changes.
Subject(s)
Animals , Antineoplastic Agents/isolation & purification , Cytotoxins/isolation & purification , Humans , Lacticaseibacillus casei/metabolism , Tumor Cells, CulturedABSTRACT
Forty four cultures of Lactobacilli isolated from their natural sources such as dahi, raw milk and fermenting rice-pulse doughs etc. along with four standard strains of Lactobacilli were assayed for their cytotoxic activity against three secondary tumour cell lines. Three cultures isolated from dahi samples and identified as Lactobacillus casei D-34, L. casei D-48a, L. plantarum D-70a along with one standard strain L. casei B 1922 exhibited significant cytotoxic activity in the range of 30 to 36%.