ABSTRACT
A rapid, simple, selective, precise, and accurate stability indicating HPLC method has been developed and validated for the simultaneous analysis of esomeprazole and itopride in bulk and in capsule form. An isocratic separation was achieved using a Hypersil C4 (250 x 4.6 mm), 5 μm particle size column with a flow rate of 1 mL/min and photodiode array detector at 272 nm. The mobile phase consisted of 0.1M dipotassium hydrogen phosphate: acetonitrile (40:60 v/v). The method was validated for selectivity, specificity, linearity, precision, accuracy and robustness. The selectivity of the method was determined by assessing interference from the placebo, components of mobile phase and common excipients in pharmaceutical formulations. Whereas, specificity was established by stress degradation studies. The method was linear over the concentration range 40–120 μg/mL (R2 = 0.9999) and 150-450 μg/mL (R2 = 0.9999) for esomeprazole and itopride, respectively. Limit of detection is 0.207 and 0.724 μg/mL & Limit of quantitation is 0.691 and 2.415 μg/mL for esomeprazole and itopride, respectively. The precision and accuracy of the method was found to be acceptable. The method was found to be robust and suitable for the simultaneous analysis of esomeprazole and itopride in a capsule formulation. Degradation products resulting from the stress studies did not interfere with the detection and quantification of esomeprazole and itopride. The proposed HPLC method is thus stability-indicating.
ABSTRACT
Three Activated Kaza’s Carbons viz. NPTC, NLLC andNCC, are prepared from some Bio- Materials – Phaseolus trilobus, Leucena leucocephala and Casurina. These carbonsare used to adsorb dyes Methylene blue and Rhodamine B from aqueous solution. Adsorption equilibrium data and kinetic processes were studied. The Langmuir isotherm provides the best correlation for the experimental data, where as Temkin and Dubinin – Radushkevich (D-R) isotherms also fit to the experimental data. In the kinetic studies the best fit Model was assessed by the squared some of errors (SSE) values. Adsorption of dyes, Methylene Blue ( MB ) and Rhodamine B ( RB ) ,on to adsorbents could be described by pseudosecond order equation. The adsorption process has good co-ordinance with pore diffusion and Elovich equations.
ABSTRACT
A 55-year-old woman presented with fever, breathlessness and shock. She was diagnosed to have diabetes mellitus (Type 2) after admission. Blood culture grew Burkholderia pseudomallei. The patient responded to intravenous ceftozidime for two weeks and a prolonged course of six months with cotrimoxazole and doxycycline.
ABSTRACT
Prepared three different activated carbons from parts of three different bio-materials viz., Phaseolus trilobus, Leucena leucocephala and Casuarina collected from agricultural field. In the present study phsicochemical and surface characterstics of these prepared carbons have been discussed. For surface characterization FTIR and EDAX methods were used.