ABSTRACT
Recent attention has been directed towards the role of mast cells in the pathogenesis of oral lichen planus. Mast cells are responsible for trafficking of inflammatory cells into the connective tissue that in turn helps in progression and maintenance of the chronicity of oral lichen planus. In the present study we have evaluated the mast cell count and its cell types using toluidine blue stain, in oral lichen planus and compared it with oral lichenoid reactions. We have observed an increase in mast cell count in both these conditions in comparison to normal mucosa. Also, a significant difference in the types of mast cells in oral lichen planus and oral lichenoid reactions has been noted, possibly suggesting that the role of mast cell is different in these conditions.
Subject(s)
Cell Count , Cell Degranulation/physiology , Cell Nucleus/ultrastructure , Chronic Disease , Coloring Agents/diagnosis , Connective Tissue/pathology , Cytoplasmic Granules/ultrastructure , Disease Progression , Humans , Lichen Planus, Oral/pathology , Lichenoid Eruptions/pathology , Mast Cells/classification , Mouth Mucosa/pathology , Statistics, Nonparametric , Tolonium Chloride/diagnosisABSTRACT
Oral lichen is a mucocutaneous disorder, which presents a confusing array of patterns and forms. Despite the reported differences between the classical lichen planus and lichenoid reaction, several reports have concurred with this lack of distinguishing features. As of yet there is no specific test for lichenoid reaction, although resolution and recurrence of lichenoid reaction on withdrawal and exposure to the drug is probably diagnostic. Thus when the clinical and histopathologic evaluation cannot confirm the diagnosis satisfactorily, the use of immunofluorescent examination is of great importance. Early diagnoses of these conditions are more likely with adjunctive use of immunofluorescent examination. Further, this tool may provide an insight into the pathogenesis of these disorders.
Subject(s)
Antigens/analysis , Biomarkers , Fluorescent Antibody Technique , Humans , Lichen Planus, Oral/diagnosis , Lichenoid Eruptions/chemically inducedABSTRACT
Immunoglobulin synthesizing plasma cells for IgG were quantitated in 20 periapical granulomas and 20 periapical cysts, using unlabelled antibody peroxidase-antiperoxidase complex method. Result showed that immunoglobulin G producing plasma cells were predominant in periapical cyst as compared with periapical granuloma. A statistical significant relation was observed between these two lesions.
Subject(s)
Humans , Immunoenzyme Techniques , Immunoglobulin G/biosynthesis , Periapical Granuloma/immunology , Plasma Cells/metabolism , Radicular Cyst/immunologyABSTRACT
Glandular Odontogenic cyst is an apparently rare jaw cyst characterised by typical histopathological features, propensity to reach large size and high rate of local recurrence, if not adequately treated. Identification of this cyst as a separate entity is important because of the difference in biological behaviour. We report a case of Glandular Odontogenic cyst occurring in maxilla.
Subject(s)
Adolescent , Connective Tissue/pathology , Epithelium/pathology , Facial Asymmetry/diagnosis , Female , Humans , Maxillary Diseases/diagnosis , Mucous Membrane/pathology , Odontogenic Cysts/diagnosisSubject(s)
Adult , Aged , Aged, 80 and over , Analysis of Variance , Areca , Carcinoma, Squamous Cell/genetics , Confidence Intervals , DNA Damage , Female , Fluorescent Dyes/diagnosis , Humans , Male , Micronuclei, Chromosome-Defective/genetics , Middle Aged , Mouth Neoplasms/genetics , Plants, Medicinal , Plants, Toxic , Smoking , Statistics as Topic , Tobacco, Smokeless , Biomarkers, Tumor/geneticsABSTRACT
Cementum in human tooth is a hard tissue in its root deposited around dentin in layers throughout the life. Microscopically each layer is seen as a set of alternating dark and light bands and called as Cemental Annulations. Intact teeth obtained from subjects of either sex and of known ages processed by ground sectioning manually and mounted on a glass slide. The cemental annulations were then counted by light microscope using CCTV Screen, at the junction of cervical with middle third of root. Age was then determined by adding the eruption age in years of tooth in study to the annulations counted. This was found to be matching with actual age almost to an accuracy of +/- 1-2 years.