ABSTRACT
OBJECT: Two easy methods for the diagnosis of h pylori i.e: Serum Anti H Pylori Antibodies and Stool Antigen
MATERIALS AND METHODS: Hundred serum and stool samples collected from the department of surgery, and Medicine were registered at Department of Pathology and Diagnostic and Research Lab LUMHS, Jamshoro/ Hyderabad for performing H. pylori diagnosis were included in this Study. Patients of all age groups and both sexes with complain of dyspepsia, or having the linical suspicion of H. pylori associated gastritis were included. Patients having H/O NSAIDs, or eradication therapy were excluded. A specially designed proforma was used for recording the relevant data. The Period of study was 15-10-2009 to 28-02-2011
RESULTS: From 100 cases of, H. pylori antibodies were found in 68 [68 %]. 38 [55.9%] were male and 30 [44.1%] were females. Sensitivity of this technique was 75.55% and specificity 60%. P value 0.007. From stool samples collected from 100 patients H. pylori antigen were positive in 48 [48%] cases detected on immunochromatography technique. Stool antigen positive was in 28 [58.3%] males and 20 [41.7%] Female patients. The stool antigen sensitivity was 52.74% and specificity 90% P value [0.011]
CONCLUSION: H. pylori positivity is highly significant in histological examination of endoscopic biopsies on H and E and Giemsa Stains The detection of anti H. pylori antibodies from serum by ELISA method and the H. pylori antigen from stool samples by immunochromatographies method, can be non invasive simplest, least expensive and alternatives in patients unwilling for endoscopy
ABSTRACT
OBJECT: To find out the frequency of H.pylori infection in patients undergoing upper GI endoscopy
MATERIALS AND METHODS: This study was conducted at surgical unit-I Liaquat University Hospital and private hospital located at Hyderabad Sindh from June 2010 to July 2011. After taking written consent those Patients were enrolled who fulfilled the inclusion criteria. Study was approved by ethical committee. Presence of H.pylori infection was confirmed by histopathological examination of samples taken during upper gastrointestinal endoscopy. Presence or absence of gastritis was also observed. GIF 140 Olympus gastro-video-scope was used for the procedure
RESULTS: 165 patients were enrolled in the study, among them 82 were males and 83 females with mean age 41.04 +/-15.9 and 35.9 +/-11.5 years respectively, among males 43 [52.43%] were positive and 39 [47.5%] were negative for H.pylori infection, while in females 38[45.78%] were positive and 45 [54.2%] negative for the same. When compared for gender differences there was slightly higher prevalence in males. Antral Gastritis was common association with H.pylori infection in both genders with higher prevalence in males as compared to females. 5 patients [5%] were found to have gastric carcinoma without any H.pylori association CONCLUSION: Helicobacter pylori infection is quite frequently found in association with gastritis in males as well as females with slightly higher prevalence in male