ABSTRACT
Background: Chlamydia trachomatis [CT] infection is the most common sexually transmitted disease in the world that can persist and also ascend in the genital tract. This intracellular and silent infection is related to some adverse pregnancy outcomes, such as miscarriage. The aims of this study were to explore the best CT screening tests using blood and vaginal samples and to investigate the correlation between CT infection and the incidence of miscarriage
Materials and Methods: This case-control study was done in October 2013 through June 2014, using purposive sampling from 157 female participants with or without a history of miscarriage. The samples were taken after each participant had signed a letter of consent and had completed a questionnaire. To achieve the objectives of this study, polymerase chain reaction [PCR] and enzyme-linked immunosorbent assay [ELISA] tests were performed on vaginal swabs and blood samples, respectively
Results: PCR results showed a significantly higher CT infection rate in the miscarriage group compared to the control group [11.3 vs. 0%, P=0.007]. Anti-CT IgG and IgA antibodies were found in 4.2 and 2.1% of cases in the miscarriage group, and in 1.7 and 6.7% of cases in the control group, respectively [P>0.05]. Despite lower humoral responses in this study, positive samples were detected only by one of the following techniques; PCR, ELISA IgA and ELISA IgG. It also should be noted that PCR worked best in terms of detection
Conclusion: Based on the obtained data, there is a strong association between molecular evidence of CT infection and miscarriage. A higher rate of CT detection in molecular tests compared to serological assays suggests that PCR could be used as the first-choice assay for detection of C. trachomatis. However, the importance of serological tests in detecting potential past CT infection or upper genital infection not amenable to sampling is undeniable
ABSTRACT
Background and Aims: infectious mononucleosis [IM]is the clinical manifestation of primary infection with Epstein-Barr virus [EBV]. Humans are the only known reservoir of EBV. Regarding the problems in diagnosis of the disease, the purpose of this study was to assess Enzyme-linked immunosorbent assay [ELISA] and Nested polymerase chain reaction [PCR] as a diagnostic tool for this disease
Materials and Methods: 50 samples were collected from the suspicious patients with EBV and 50 samples from the healthy individuals as the control and both techniques were applied for them
Results: the results showed that 76% of the patients and 14% of the control samples had EBV DNA in serum with PCR. Statistical analysis showed significant difference between the patient and the control samples for infection with EBV [P < 0.0001]. Samples were classified into three groups according to the ELISA that were acute phase [20%], recent infection or convalescence phase [14%] and past infection [66%], respectively
Conclusions: comparing the two methods, the results of the ELISA test indicated that ELISA would be the best method to be used for the diagnosis of IM. Our results suggest that serology may be more sensitive and could be performed as the initial screening test for acute EBV infection. Although, the PCR test is routinely used as an accurate method for detection of the pathogens with a higher specificity and sensitivity comparing the immunoassay, in IM, ELISA seems to be the best method for detecting antibodies against EBV
ABSTRACT
Chlamydia trachomatis [C. trachomatis] is the most prevalent cause of bacterial sexually transmitted infections [STI] recognized throughout the world. The aim of this study is to determine different genotypes of genital C. trachomatis and the association between the serological markers of inflammation and genotypes of C. trachomatis in sexually active women [n=80] attending Shahid Beheshti Hospital in Isfahan, Iran. In this descriptive study, endocervical swabs were collected from 80 women. There were 17 endocervical samples that showed positivity for C. trachomatis by plasmid polymerase chain reaction [PCR] using KL1 and KL2 primers. The omp1 gene was directly amplified in 17 plasmid PCR positive samples and was used to differentiate the clinical genotypes by omp1 gene PCR-restriction fragment length polymorphism [PCR-RFLP]. The levels of IgG and IgA specific to C. trachmatis and C-reactive protein [CRP] were evaluated. Based on restriction-digestion patterns, four genotypes were identified. Genotypes E [35.3%] and F [35.3%] were the most prevalent, followed by D/Da [23.5%] and K [5.9%]. There was no significant association between genotypes and the presence of IgG and CRP. Patients infected with genotype E showed a serological marker of chronic inflammation, i.e. IgA seropositivity, significantly more than patients infected with other genotypes [p=0.042]. Nested PCR could increase the sensitivity of omp1 amplification. Based on the presence of IgA, chronic C. trachomatis infections were observed more frequently among genotype E-infected patients in our population