Mouse bone marrow-derived mast cells induce angiogenesis by tissue engineering in rats: histological evidence

Objective: therapeutic angiogenesis is employed to induce vascular network formation and improve functional recovery in ischemia. The aim of this study is to find an appropriate method to recover local ischemic conditions

Materials and Methods: in this experimental survey, 20 male Wistar rats weighing approximately 200-250 g were randomly divided into four experimental groups respectively: ischemia group in which the femoral artery was transected; phosphate buffer solution group [PBS] in which the femoral artery transected location was immersed with PBS; chitosan [CHIT] group in which the transected location was immersed in a 50 microL CHIT solution; and mast cell transplanted group in which the transected location was immersed with a mixture of 50 microL CHIT and 50 microL PBS that contained 1×10[6] mast cells

Results: on day 14 after surgery, mean numbers of blood vessels of different sizes in the CHIT/mast cell group significantly increased compared to the other experimental groups [P<0.05]

Conclusion: our data suggest that mast cell reconstitution could offer a new approach for therapeutic angiogenesis in cases of peripheral arterial diseases

Local xenotransplantation of bone marrow derived mast cells [BMMCs] improves functional recovery of transected sciatic nerve in cat: a novel approach in cell therapy

Objective: To determine the effects of bone marrow derived mast cells [BMMCs] on functional recovery of transected sciatic nerve in animal model of cat

Method: A 20-mm sciatic nerve defect was bridged using a silicone nerve guide filled with BMMCs in BMMC group. In Sham-surgery group [SHAM], the sciatic nerve was only exposed and manipulated. In control group [SILOCONE] the gap was repaired with a silicone nerve guide and both ends were sealed using sterile Vaseline to avoid leakage and the nerve guide was filled with 100 microL of phosphate-buffered saline alone. In cell treated group [[SILOCONE/BMMC] the nerve guide was filled with 100 microL BMMCs [2× 106 cells/100 microL]. The regenerated nerve fibers were studied, biomechanically, histologically and immunohiscochemically 6 months later

Results: Biomechanical studies confirmed faster recovery of regenerated axons in BMMCs transplanted animals compared to control group [p<0.05]. Morphometric indices of the regenerated fibers showed that the number and diameter of the myelinated fibers were significantly higher in BMMCs transplanted animals than in control group [p<0.05]. In immunohistochemistry, location of reactions to S-100 in BMMCs transplanted animals was clearly more positive than that in control group

Conclusion: BMMCs xenotransplantation could be considered as a readily accessible source of cells that could improve recovery of transected sciatic nerve

Ethyl pyruvate ameliorates the damage induced by cyclophosphamide on adult mice testes

Background: Cyclophosphamide [CP] is a chemotherapy drug which causes deleterious effects on testicular tissue and increases free radicals in the body. The aim of this study is to investigate the protective effects of ethyl pyruvate [EP] on testicular improvement in CP treated animals

Materials and Methods: In this experimental study, 15 male mice [6-8 weeks] were divided into 3 groups. The control group received normal saline [0.1 ml/day] intraperito-neal [IP], CP group received CP [15 mg/kg/week, IP], and the CP+EP group received EP [40 mg/kg/day, IP] plus CP. After 35 days, we assessed serum total antioxidant capacity [TAC] along with histomorphometric and histochemical analyses of the testicles

Results: The mean thickness of the germinal epithelium, diameter of seminiferous tubules, and the number of Leydig cells in the CP+EP group were higher than those of the CP group [P<0.05]. The number of the mast cells in the CP+EP group significantly reduced compared with the CP group [P<0.05]. Alkaline phosphatase [ALP], periodic acid-schiff [PAS] positive reactions and lipid granules in cytoplasm of the Leydig cells in the CP group increased compared with the other groups [P<0.05]. TAC in the CP group significantly reduced compared with the other groups [P<0.05]

Conclusion: This study showed the ability of EP to reduce the destructive side effects of CP in the adult mice reproductive system

Protective effects of ethyl pymvate on sperm quality in cyclophssgphamide treated mice

AbstrnetBackground: One of the affecting factors in disturbance process of spermatogenesisis chemotherapeutic-induced oxidative stress resulted from cyciophosphamide [CP]; treatment which leads to diminisled sperm quality via interference in spermatogenesis process. Objective: This study was conducted to investigate the effects of ethyl pyruvat [EP] in reducing the CP-induced side effects on reproductive system.Materials and Methods: 24 mature male mice were randomly divided into 3 equal j groups and were undergone therapy for 35 days. Control group received normalsaline [0.1 mliday, IF]. CP group were injected CP [15 mgikglweek, IP] and CP+EPgroup received EP [40 mgilcglday, IP] as well as CP. In the end of the treatment: period, the mice were euthanized by cervical dislocation. Then, the epididymis wasincubated with C02 in a human tuba1 fluid medium [1 ml] for half an hour in order: to float sperm. Then, the number, motility, viability [eosin-nigrosin staining], DNAbreakage [acridine orange staining], nucleus maturity, and sperm morphology I [aniline blue staining] were analyzed. Results: The average [15.871-1.28], motility [35.77+2.75], viability [4013.03], I nucleus maturity [36*2.79] and sperm morphology [61.751-0.85] were decreasedI significantly in CP group in comparison with control and EP groups, whereas I caused significant increase of these parameters. Also, the percentage of DNA I damage was increased significantly in CP group [41.75*3.75] in comparison with I control [2*0.71] and EP groups [22.514.13]. Conclusion: The results of this study revealed ameliorating effects of EP on sperm quality of CP treated animals