ABSTRACT
AIM AND OBJECTIVE:To know the different types of NLFs and their Antimicrobial Resistant Pattern.MATERIALS AND METHODS:This retrospective study was done from January 2012 to December 2016, in the Microbiology Laboratory of the GCRI, Ahmedabad. Different sampes like Pus discharges from post operative surgeries, absesses, flap infections, septicaemia and bacteremia, respiratory and urinary tract infections were received in the laboratory for culture and sensitivity. Standard laboratory methods were used for growth (Bactec), and automated ID AST(Vitek 2 Compact) was used for diagnosis of organism. Results: Out of 25154 samples, 48.12% (12105) bacterial growth was present including gram positive and gram negative bacteria. Out of 12105 bacterial isolation, 42.09% (5096) gram positive and 57.90% (7009) gram negative bacteria were detected. It was observed that the NLFs were quite less when compared to lactose fermenter and they accounted to 36.05% (2527/7009). Most of the NLFs, were Pseudomonas spp. 56.19% (1420) followed by next common NLF, which was Acinetobacter Spp. 34.82% (880) and others like Burkholderia spp., Stenotrophomonas spp., Achromobacter Spp. and Alcaligenes Spp. 1.22% (31) NLFs were unidentified. CONCLUSIONS:NLFs were resistant to most of the commonly used antibiotics like Beta Lactam, Beta Lactam inhibitors, Carbapenems, Aminoglycosides, Flouroquinolones. It was surprising to note that the uncommonly isolated NLF like stenotrophomonas was resistant (50-100%) to most of the antibiotics. So, the frequency of antibiotic resistant NLFs is increasing and complicating the treatment of cancer patients.