ABSTRACT
A simple, sensitive and precise high performance liquid chromatographic method for the analysis of Pantoprazole sodium and lansoprazole has been developed, validated and used for the determination of compounds in commercial pharmaceutical products. The compounds were well separated an isocratically on a C18 column [Use Inertsil C18, 5m , 150 mm x 4.6 mm] utilizing a mobile phase consisting of acetonitrile: phosphate buffer (60:40, v/v, pH 7.0) at a flow rate of 1.0 ml/min with UV detection at 230 nm. The retention time of Pantoprazole sodium and lansoprazole was found to be 2.017 min and 2.538. The procedure was validated for linearity (Correlation coefficient = 0.999). The study showed that reversed-phase liquid chromatography is sensitive and selective for the determination of Pantoprazole sodium and lansoprazole using single mobile phase.
ABSTRACT
Sheath blight of rice caused by Rhizoctonia solani is an economically important disease affecting rice production. Eight Pseudomonas fluorescens strains were isolated from rhizosphere of rice seedlings collected from Andhra Pradesh and Tamilnadu. These strains were characterized with PCR based RAPD technique and tested for their in-vitro antagonistic activity against R. solani. Crude metabolites from one particular isolate of P. fluorescens (P. f 003) were extracted with organic solvents such as ethyl acetate and petroleum ether and these were tested against R. solani. Commonly used fungicides in rice sheath blight disease management such as hexaconazole, carbendazim, copper oxy chloride and mancozeb at 150 ppm were screened against mycelial growth of R. solani using poisoned food technique. All the strains tested were exhibited antagonistic activity against R. solani. One isolate, P.f 003 gave 78% inhibition compared to control. All the fungicides and crude extracts of P.f.003 inhibited the mycelial growth of R. solani. Highest inhibition was recorded with hexaconazole and ethyl acetate crude metabolite extract. The results offer a scope for integrating P. fluorescens with chemical fungicides for control of sheath blight of rice.
ABSTRACT
Rice blast caused by Magnaporthe grisea and sheath blight caused by Rhizoctonia solani are the major diseases affecting the rice production. Application of beneficial bacteria as seed or seedling root dip to protect these diseases may be an alternative strategies to chemical control. In this study, fluorescent Pseudomonads isolated from rice seedlings were used to screen for their antagonistic ability and siderophore mediated antibiosis under in-vitro conditions against these pathogens. Among 10 isolates, strain P.f 003 gave significantly higher inhibition of mycelial growth of M. grisea and R. solani. Strains of P.f 001, P.f 003, P.f 005 and P.f 007 produced siderophores when grown on Fe deficient and Fe fortified King’s B medium. These strains again tested for their in-vitro antagonistic activity against M. grisea and R. solani on King’s B media with or without FeCl3. Our results showed that all these strains significantly reduced the growth of M. grisea and R. solani with FeCl3 in the media compared to without FeCl3. Strain P.f 003 activity was superior compared to other strains evaluated.
ABSTRACT
A simple, selective, accurate reverse phase-high Performance Liquid Chromatographic (RP-HPLC) method was developed and validated for the analysis of Sildenafil Citrate in pharmaceutical formulations. Chromatographic separation achieved isocratically on a C18 column [Use Inertsil C18, 5m , 150 mm x 4.6 mm] utilizing a mobile phase of acetonitrile/phosphate buffer (70:30, v/v, pH 7.0) at a flow rate of 0.8 ml/m with UV detection at 228 nm. The retention time was 4.087. The method is accurate (99.15-101.85%), precise (intraday variation 0.13-1.56% and inter-day variation 0.30-1.60%) and linear within range 0.1- 30μg/ml (R2=0.999) concentration and was successfully used in monitoring left over drug. The detection limit of sildenafil citrate at a signal-to-noise ratio of 3 was 1.70ng/ml in formulations while quantification limit in drug was 5.40 ng/ml. The proposed method is applicable to stability studies and routine analysis of sildenafil citrate in pharmaceutical formulations.