ABSTRACT
To see the possible gastro protective effect of Sagu pearls on Diclofenac Sodium [NSAID] induced gastric ulcer by enhancing mucosal barrier. Randomized control trial. The study was carried out in the department of Anatomy, Islamic International Medical College, Rawalpindi, in collaboration with National Institute of Health, Islamabad. It was conducted for a period of six months, from 15th September 2014 till 30th March, 2015. Fifty adult rats of both sexes of Sprague Drawly strain were divided into three groups: Group I [control]; Group II [ulcer group] given Diclofenac sodium orally at the dose of 50mg/kg body weight, daily for 2 weeks and Group III given Sagu pearls daily at the dose of 200mg/kg body weight for 2 weeks along with Diclofenac Sodium. Animals of all the groups were sacrificed on day 16 and their stomachs were studied macro and microscopically. Statistical analysis was done to see any significant difference between the groups. Anti-ulcer effects were assessed on the qualitative and quantitative parameters like ulcer size and index, histological determination of depth of the mucosal lesion and mucus thickness. Results highlighted the probable protective effect of Sagu pearls by exhibiting almost 90% decrease in ulcer index in group III accompanied by a continuous thick mucus layer on the surface of mucosal cells confirmed by Periodic Acid Schiff [PAS] stain. Sagudana can provide protection against NSAID induced gastric ulcer by strengthening mucus barrier and thus can be used as an adjunct along with NSAIDs
ABSTRACT
To determine the effects of ginger extract on the histomorphological changes of fatty liver of hyperlipidemic diet induced NAFLD. It was randomized control trial. The study was carried out from October 2012 to March 2013, in the department of Anatomy, Islamic International Medical College, Rawalpindi, in collaboration with National Institute of Health [NIH], Islamabad. A total of 35 male albino mice were used and divided into 3 groups. The control group [C] was fed on normal laboratory diet, while the remaining two groups, fatty group [FG], ginger group [GN], were fed on hyperlipidemic diet for twelve weeks to induce hyperlipidemia/nonalcoholic fatty liver. Then GN group with induced hyperlipidemia/ fatty liver was administered normal laboratory diet with ginger extract as a drink in replacement of water for another twelve weeks. Total body weight was reduced as compared to their initial body weights. The histological examinations of this study revealed reverse fatty [steatotic] changes and showed marked reduction in number of fat globules, ballooning degeneration, glycogenated nuclei. This research shows that ginger extract has marked antihepatotoxic effects. Ginger extract ameliorates high fat induced fatty liver disease
ABSTRACT
To determine the comparative histological effects of Shish a and cigarettes on trachea of experimental animals. It was an analytical experimental randomized control trial. The study was carried out in the department of Anatomy, Islamic International Medical College, Rawalpindi, in collaboration with National Institute of Health [NIH], Islamabad. The duration of the study was 6 months. 40 adult male BALB/c mice were used and divided into 3 groups. Control group C was kept in a chamber in fresh air. Group 55 was exposed to Shisha smoke and Group CS was exposed to equivalent quantity of nicotine by burning cigarettes. All mice were dissected after 8 weeks and tissues of trachea were examined microscopically and results were compared in experimental groups. The trachea was examined for three parameters. There was marked mucus cell hyperplasia and submucosal gland hypertrophy in group SS and the difference between group 55 and CS was statistically significant [p