ABSTRACT
Background: Atherosclerosis has been considered as the disease of intima with the role of tunica adventitia so far neglected. Current evidence shows that adventitia, and particularly the vasa vasora [VV] reacts to the process of atherogenesis
Objective: The study was aimed to calculate the VV density in adventitia and to correlate it with intimamedia thickness in aorta of cholesterol-fed rabbits
Materials and Methods: This experimental study was conducted in Army Medical College Rawalpindi. Samples of vessels were obtained from twenty adult NZW rabbits fed normal [group A] or 2% high-cholesterol [group B] diet for 6 weeks [n=10/group]. Aortic sections were taken from each part [ascending, arch, descending thoracic and abdominal] of every aorta. Light microscopic cross sectional analysis was performed in H andE stained slides. Intima-media thickness [IMT] and adventitial VV density were calculated and their correlation was investigated in each part of aorta
Results: A total of 80 cross sections were analyzed. Mean+/-SE IMT and adventitial VV density was significantly increased in ascending, arch, and descending thoracic parts of group B versus their equivalent aortic parts in group A. But, there was an insignificant difference of means regarding VV density of abdominal aorta between both groups, despite a significant increase of IMT in group B. A very strong positive correlation was observed between the adventitial VV density and IMT in each aortic part of cholesterol-fed rabbits
Conclusion: The present study showed that increased IMT is strongly associated with enhanced adventitial VV density in each aortic part of cholesterol-fed rabbits
ABSTRACT
The objective of this study was to observe the morphometric changes in the testes of albino rats exposed to lead acetate. Experimental animal study. This study was conducted from January to April, 2009 at National Institute of Health Islamabad. Animals were obtained from the animal house of N.I.H and were divided into two groups A and B. The animals in group A were used as control, while those of groups B were treated with lead acetate that was given intraperitonially in the dose of 4mg/kg body weight, 5 days a week for 6 weeks. After 6 weeks the animals of group A [control] and group B [experimental] were sacrificed by an overdose of ether anesthesia. The testes were fixed in formalin and then processed for paraffin embedding. Five micrometer thick section were cut, stained with hematoxylin and eosin, and observed microscopically for germinal epithelium thickness and the basement membrane of seminiferous tubules. The histological comparison of testes of both groups of animals showed that after six weeks, the width of germinal epithelium and the number of spermatogenic cells had decreased in the test group as compared to the control group [p<0.05] and in majority of the seminiferous tubules, the basement membrane was disrupted
ABSTRACT
To investigate the variations in the total number of segmental arteries in human kidneys obtained from cadavers of the local adult population. Observational Study. This study was conducted at Anatomy Department of University of Health Sciences, Lahore, for a period of one year from October 2006 to October 2007. Forty four adult human kidneys were obtained after autopsy; they were randomly divided in two groups A and B of right and left kidneys respectively. Simple blunt dissection and corrosion cast techniques were used to study the number of segmental arteries. Statistical analysis was carried out by using SPSS version 16.0 and STATA version 8.0. Segmental arteries were present in 100% of specimens of groups A and B; variations were seen in the number of segmental arteries of both groups. The segmental branches of renal artery in local population showed variations different from those reported in the earlier work carried out in other countries