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Objective To study high-quality and high efficient meshing methods for skeleton. Methods Based on characteristics of bone structure, the mesh generation technology of computational fluid dynamics (CFD) was applied in mesh generation for bone biomechanics. Hexahedron elements and tetrahedron elements were used to simulate cortical bone and cancellous bone, respectively. Results By using CFD mesh generation technology, high-quality hexahedral elements could be obtained, the structure characteristics of skeleton could be preferably simulated, and computer automatic meshing could be implemented. The amount of time for meshing bones by the new method was only about 1/5 of that by the traditional method, and the calculation results obtained from the finite element model were basically consistent with the results from the cadaver experiment. Conclusions The CFD mesh generation technology can be applied in the field of bone biomechanics, which provides an effective approach to reconstruct complex human skeleton.
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Objective To study high-quality and high efficient meshing methods for skeleton.Methods Based on characteristics of bone structure,the mesh generation technology of computational fluid dynamics (CFD) was applied in mesh generation for bone biomechanics.Hexahedron elements and tetrahedron elements were used to simulate cortical bone and cancellous bone,respectively.Results By using CFD mesh generation technology,high-quality hexahedral elements could be obtained,the structure characteristics of skeleton could be preferably simulated,and computer automatic meshing could be implemented.The amount of time for meshing bones by the new method was only about 1/5 of that by the traditional method,and the calculation results obtained from the finite element model were basically consistent with the results from the cadaver experiment.Conclusions The CFD mesh generation technology can be applied in the field of bone biomechanics,which provides an effective approach to reconstruct complex human skeleton.
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<p><b>OBJECTIVE</b>To study the methylation level in the promoter of caspase 8 associated protein 2 (CASP8AP2) gene between samples at diagnosis and in complete remission, and to investigate its relationship with clinical features and prognosis in children with acute lymphoblastic leukemia (ALL).</p><p><b>METHODS</b>Diagnostic DNA samples from 109 newly diagnosed children with ALL admitted from August 2007 to March 2010, and 94 ALL children in CR (complete remission) among them were collected. Bisulfite modification and MethyLight method established by our research team were used to determine the methylation level of the two key CpG sites (at -1189 and -1176) of the promoter of CASP8AP2 gene.</p><p><b>RESULTS</b>The average methylation level of the two CpG sites in newly diagnosted samples was higher than that in CR samples (71.1% ± 1.7% vs 64.2% ± 21.2%) (P = 0.008). Analysis with receiver operating characteristic (ROC) curve showed that the area under curve was 0.687 (P = 0.024), indicating that the methylation level of the two CpG sites was able to predict relapse efficiently to some extent, 76.9% was chosed as a cutoff value to divide the patients into high methylation group (49 patients) and low methylation group (60 patients). The incidence of relapse in high methylation group was higher than that in low methylation group (20.4% vs 6.7%) (P = 0.044), five year relapse free survival in high methylation group was also lower than that in low methylation group (Log rank, P = 0.033). Furthermore, high methylation at new diagnosis were correlated with high level of minimal residual disease (MRD) before consolidation therapy (P = 0.011). In the 34 children with MRD ≥ 10(-4) at the end of induction remission, the relapse rate of high methylation patients was significantly higher than that of low methylation patients (8/16 vs 3/18)(P = 0.038).</p><p><b>CONCLUSION</b>The abnormal hypermethylation of the two CpG sites (at -1189 and -1176) of the promoter of the CASP8AP2 gene is possibly associated with leukemogenesis in childhood ALL. The treatment outcome is more poor in patients with hypermethylation than that in patients with low methylation. The combination of the methylation level of the two CpG sites and MRD level at the end induction remission is able to predict relapse more effectively.</p>
Subject(s)
Child , Humans , Apoptosis Regulatory Proteins , Calcium-Binding Proteins , DNA Methylation , Neoplasm, Residual , Precursor Cell Lymphoblastic Leukemia-Lymphoma , Prognosis , Promoter Regions, Genetic , Recurrence , Remission InductionABSTRACT
Objective To provide the evidence for health management decision-making and rational use of drugs grass root clinics by studying their drug prescription rules.Methods The prescribed drugs in clinics of 5 township health centers from September 2012 to September 2014 were retrieved from The Management Information System of Sichuan Grass Root Medical Institutions.Their big data were analyzed using R language.Results The commonly pre-scribed drugs in clinics were vitamin B6, vitamin C and cefixime tablets, which were usually used in combination. Conclusion Health administrative organizations can strengthen their supervision and management of prescribed drugs and promote their rational use in grass root clinics using unified management information system of grass root medical institutions in combination with information technology .
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The medical and health organization management information system at grass-root level and platform at county level for data exchange in Sichuan Province were designed and constructed according to the health information exchange service network in Sichuan Province and standard medical CDA file, in order to implement data exchange on the medical and health organization management information system at grass-root level and platform at county level, to insure the basic medical and health service for the public, and to improve their health level.
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After a description of the background and ideas to introduce the third party testing in information system construction of Sichuan grass-root medical and health institutions and the specific working contents of third party testing team, the problems to which importance should be attached in information system construction were analyzed with suggestions put forward for their solution .
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After a description of the basic contents of Sichuan grass-root health information, health information systems at county level or below, and their roles and features, the key points of Sichuan grass-root health informa-tion construction program, progress and problems in Sichuan grass-root health information construction were stressed in discussion , with certain measures put forward for the solution of such problems .
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The Offsite Medical Accounting Information Supervising System was developed for patients of Sichuan new rural cooperative medical scheme (NRCMS) using the C#programming language under .NET development environ-ment based on Microsoft Visual Studio 2010 in order to solve the problems in offsite medical accounting information statistics and supervision for patients of NRCMS.The system is a B/S-based MVP 3-tier structure with VPN hard-ware firewall and VPN client software plus certificate built-in, and can thus be used to supervise the offsite medical accounting for patients of NRCMS, analyze their medical advice seeking indexes at other places, and provide data for the NRCMS fund management .
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<p><b>OBJECTIVE</b>To study the APC and E-cadherin gene promoter hypermethylation as tumor marker and to investigate the correlation of free tumor-related DNA in serum and tumor tissue with clinicopathological parameters. Their feasibility in early diagnosis, predicting therapeutic effect and monitoring recurrence was evaluated.</p><p><b>METHODS</b>84 cases with operated breast cancer were recruited from March 2002 to August 2002 at Beijing Cancer Hospital. Aberrant methylation of E-cadherin and APC genes was detected in tumor tissues, adjacent normal tissues and peripheral blood serum by methylation-specific PCR (MSP). 10 cases with benign breast diseases were selected as control group.</p><p><b>RESULTS</b>The positive rate of promoter hypermethylation of E-cadherin and APC genes in tumor tissues was 52.4% and 45.2%, in the paired serum was 33.3% and 31.0%, respectively. Aberrant methylation of free DNA in serum presented the same alteration in tumor tissues. E-cadherin and APC hypermethylation in serum and tumor samples significantly correlated each other (E-cadherin P < 0.001; APC P = 0.002). The sensitivity of detection of free DNA methylation of E-cadherin and APC genes in serum was 63.6% and 63.2%, respectively. The specificity was 100% and 95.7%, respectively. There was no correlation for the aberrant methylation in cancer tissues and serum with the clinicopathological parameters of patients including age, tumor staging, tumor size, histological type and receptor. None of the aberrant methylation was found in adjacent normal tissues and control group serum.</p><p><b>CONCLUSION</b>The same aberrant methylation in cancer tissues and serum, not correlating with tumor staging, can be detected in about one third of breast cancer patients. The aberrant methylation in serum can disappear after operation. The results imply that this approach may be feasible for early diagnosis, evaluation of therapeutic effects and monitoring recurrence of breast cancers.</p>