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Objective@#To study the molecular epidemiology and genetic variation of coxsackievirus B5 (CV-B5) in certain areas in China.@*Methods@#MEGA 6.0 software was used to analyze the complete VP1 region of CV-B5 isolated strains from certain areas of China by retrieving the GenBank nucleotide database. Besides, the phylogenetic tree was constructed, the homology of nucleotide and amino acids were calculated and the rate of evolution was estimated.@*Results@#A total of 189 Chinese CV-B5 isolated strains were included in this study. Most of Chinese CV-B5 isolated strains belonged to genotype C, accounted for 90.5%. Compared with the genotype A, the homology of nucleotide sequences and amino acid sequences of complete VP1 region of 189 Chinese isolated strains were 79.8%-82.8% and 92.6%-97.9%, respectively; moreover, the nucleotide and the amino acid homology of 189 Chinese CV-B5 isolated strains among themselves ranged from 80.3% to 100.0% and ranged from 91.5% to 100.0%, respectively. The estimated rate of evolution of the CV-B5 was 4×10-3 substitutions/site/year.@*Conclusions@#The majority of CV-B5 isolated strains belonged to genotype C, and subgenotype C1 and C2 were co-circulating together in certain areas of China.
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Objective To evaluate the safety and immunogenicity of Shigella flexneri 2a conjugate vaccine.Methods A random and double blind study were carried out to compare the safety sero conversion rates and geometric mean titer(GMT)increase of Shigella flexneri 2a conjugate vaccine with phosphate-buffered saline used as control.Results The results showed that no severe systemic and local reaction rates occurred in trial group,which were not statistically significant compared with the control group.The seroconversion rate of both two weeks and twelve weeks after two doses(increased by 4 folds) were 86.27% and 79.74%;for GMT were 1∶361.83 and 1∶326.21 and increased averagely 1.08 and 0.98 times,which showed significant difference with those of the control group.Conclusion Shigella flexneri 2a conjugate vaccine was safe and its immunogenicity was good in group over 2 years old.Trialregistration National food drugs surveillance administrative bureau,"Medicine Clinical Experiment Written directive from a superior"2003L03808 number.
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Objective To finally diagnose the outbreak of acute respiratory infection caused by Adenovirus 3 in students of the north of Jiangsu province by serologic study. Methods An enzyme-linked immunosorbent assay (ELISA) has been used to detect the adenovirus IgM in the serum of acute phase and the adenovirus IgG in the serum of recovery phase. A neutralization test has been used to detect the neutralization antibody in the serum of acute and recovery phase from cases and in the serum from the control. The SPSS11.0 has been applied to the statistical analysis of the data. Results The positive rate of IgM, IgG and recovery phase neutralization antibody of cases are 3.7%, 44.4% and 59.5% respectively, while those of control are 0%, 8.3% and 33.3% respectively, and the P values of Chi-Square are 0.510, 0.018 and 0.226 respectively. The neutralization test of paired serum of 6 in 9 cases showed that the antibody titers was obviously increased. The concordance between IgG detected by ELISA and neutralization antibody detected by neutralization test is 61.4% and the P value of Kappa is 0.070. Conclusion By the serologic study, we can finally diagnose that the outbreak of acute respiratory infection was caused by Adenovirus 3.