ABSTRACT
Objective To compare the treatment efficacy and improvement of relevant indexes between electroacupuncture and biofeedback therapy in treating spastic pelvic floor syndrome (SPFS).Method Fifty SPFS patients were randomized into an electroacupuncture group and a biofeedback group by their visiting sequence, 25 cases each. In the electroacupuncture group, Baliao [Shangliao (BL31), Ciliao (BL32), Zhongliao (BL33), and Xialiao (BL34)], Zhigou (TE6), Dachangshu (BL25), Shangjuxu (ST37), Xiajuxu (ST39) and Tianshu (ST25) were selected and connected to HANS electroacupuncture apparatus, at 2 Hz and withinthe tolerable intensity, 30 min for each session. The treatment was given once every day, 6 sessions as a treatment course, with 1-day interval between two courses, for 4 courses in total. The biofeedback group was intervened by biofeedback therapy under doctor's guidance to correct the wrong defecation behavior. The treatment was given once every other day, 6 sessions as a treatment course, for 4 weeks in total. At the end of intervention, the therapeutic efficacy was evaluated, the anorectal angulation was measured by defecography (DFG), and the resting and contracting anorectal pressures were determined by anorectal manometry. Result There was no significant difference in comparing the therapeutic efficacy between the electroacupuncture group and biofeedback group(P>0.05). The anorectal angulation and pressure were improved in the two groups(P<0.05), and the improvement in the electroacupuncture group was more significant(P<0.05).Conclusion Electroacupuncture can effectively treat SPFS, equivalent to the popularly used biofeedback therapy, while electroacupuncture can produce a more significant effect in improving the anorectal angulation and pressure.
ABSTRACT
Objective To investigate the effects of ?-2-macroglobulin on the aging process of human diploid fibroblasts. Methods pIRES-A2M sense and antisense vectors were constructed and transferred into 2BS cells mediated by lipofectamine.2BS/A2Ms and 2BS/A2Ma cell lines were verified by Southern and Northern blot analysis respectively.Cell growth curve,the population doublings,cell cycle analysis,staining of senescent-associated-?-galactosidase and expression of p16 and p21 in transfected cells were measured. Results Southern and Northern blot analysis verified that the exogenous cDNAs were integrated into genomic DNA in the transfected cells.The ultmost population doublings of 2BS/A2Ms cells were slightly higher than normal 2BS cells.Cell growth curve,cell cycle analysis,staining of senescent-associated-?-galactosidase and the population doublings all revealed that 2BS/A2Ms cells demonstrated obvious difference compared with 2BS/A2Ma cells((P0.05). Conclusions The aging process of 2BS cells is influenced slightly by expression of A2M.