ABSTRACT
The current study was undertaken in order to evaluate the efficacy of BSA and chitosan nanoparticle for sustainable delivery of Quercetin. BSA and chitosan nanoparticles were prepared by coacervation process and Ionic gelation method respectively and were characterized by Scanning Electron Microscope. The average size of BSA and chitosan nanoparticles was found to be 96nm and 42.7nm respectively. Quercetin loaded BSA and chitosan nanoparticles were smooth and spherical with the average size of 317.1 nm and 44.1nm respectively. The encapsulation efficiency of the BSA and chitosan nanoparticles were calculated and it was found that the drug encapsulation efficiency was more in chitosan than in BSA. The in vitro analysis of Quercetin loaded BSA and chitosan nanoparticles were studied using dialysis bag. The nanoformulation of Quercetin loaded BSA nanoparticles shows a sustainable release of drug in which 56% of the drug was released till 24 hours and it did not change further. For Quercetin loaded chitosan nanoparticles it shows 72% of in vitro drug release for 24 hours and found to be better than BSA nanoparticle. Hence both Quercetin loaded BSA and chitosan nanoparticles were effective in encapsulation and sustainable release which also paves way for the bio availability of Quercetin
ABSTRACT
To study the effect of 5-Fluorouracil (5-FU) in glaucoma filtration surgery, 13 eyes of 12 patients with glaucoma were subjected to trabeculectomy with intraoperative one minute exposure of 50 mg/ml 5-FU. The average age of patients was 36.42 +/- 18.78 years. Two of the patients had developed hypotony in the fellow eye following the use of Mitomycin C with trabeculectomy. The mean follow-up period was 9.54 +/- 5.17 weeks. Two patients developed a shallow anterior chamber with choroidals postoperatively which responded to conservative treatment. One patient developed an encysted bleb one month after surgery. Single one minute intraoperative exposure to 5-FU is a convenient and inexpensive method which appears to have no significant side effects. It may be a useful adjunctive treatment to optimise the results of glaucoma filtration surgery particularly in young and myopic patients. The long term effects, however, are not known.