ABSTRACT
<p><b>OBJECTIVE</b>To study Xionggui nasal sprays and its evaluation in release in vitro and absorption in vivo.</p><p><b>METHOD</b>Establishing the best prescription of Xionggui nasal sprays through orthogonal design methods, The in vitro release action of Xionggui nasal sprays was studied using dynamic dialyse method. The in vivo rat nasal recirculation methods were used to study the rule of Xionggui nasal sprays absorption.</p><p><b>RESULT</b>The optimum prescription was: Pemulen TR-1 0.35%, EDTA 0.2%, PEG400 1%, xanthan gum 0.2%; trolamine: right amount(adjust pH). Its release in vitro and absorption in vivo meet to Higuchi distribution.</p><p><b>CONCLUSION</b>The preparation method of Xionggui nasal sprays was appropriate. The release of drug and its uptake was well correlated.</p>
Subject(s)
Animals , Female , Male , Rats , Absorption , Administration, Intranasal , Aerosols , Angelica sinensis , Chemistry , Drug Carriers , Drug Combinations , Drug Compounding , Methods , Drugs, Chinese Herbal , Pharmacokinetics , Edetic Acid , Emulsions , Ethanolamines , Ligusticum , Chemistry , Nasal Mucosa , Metabolism , Plants, Medicinal , Chemistry , Polyethylene Glycols , Polysaccharides, Bacterial , Rats, Sprague-DawleyABSTRACT
<p><b>OBJECTIVE</b>To study the preparation of solid lipid nanoparticles loaded with Xionggui powder-supercritical carbon dioxide fluid extraction and their evaluation in vitro release.</p><p><b>METHOD</b>To prepare solid lipid nanoparticles (SLN) loaded with Xionggui powder-supercritical carbon dioxide fluid extraction (XG-CO2-SFE) using a hot dispersion- ultrasonic technique, establishing the best prescription of XG-CO2-SFE-SLN through orthogonal design methods using entrapment efficiency of nanoparticles as index, and investigating their physicochemical characterizations. The invro release action of SLN was studied in different dissolution mediums using dynamic dialyse method.</p><p><b>RESULT</b>The best prescription was: phospholipid: F-68: stearie acid glyceride = 5: 2 : 1, the entrapment efficiency of nanoparticles was 96.3%, and the results revealed the nanoparticles were sphere like with the mean size of 245.8 nm, the mean Zeta potential was -33.5 mV. The in vitro release meet to Weibull distribution in physiological brine and to single-index model in pH 7.4 phosphate liquid (40% EtOH).</p><p><b>CONCLUSION</b>The preparation method of the XG-CO2-SFE-SLN was appropriate, and the XG-CO2-SFE-SLN was released completely.</p>