The measurement of albumin in urine by the bromcresol green method compared with the results of albustix and robert’s test.

For determination of albumin in urine, Bromcresol Green (BCG) was used as follows: stock BCG regent was diluted to 1:20, and 2.5 ml. of the reagent was added to 100 µl of specimen; the resulting reaction mixture was measured for optical density at 630 nm. The values of the routine Rebert’s Test and reagent strip (Albustix) were compared to those produced by the BCG method. The mean albumin values of the BCG method were 22.4, 54.6, 95.6, 180.4, 396.3 and 1,176.3 mg/dl which were comparable to the negative, trace, 1+, 2+, 3+, 4+ values of the Robert’s Test, respectively. The mean albumin values of the BCG method were 26.3, 52.7, 96.3, 191.7, 476.1 and 1,273.1 mg/dl which were comparable to the negative, trace, 1+, 2+, 3+, 4+ values of the Albustix, respectively. The albumin values from Albusix and the Robert’s Test could be estimated from this study.

A modified micromethod for determination of albumin in cerebrospinal fluid and urine.

In this modified micromethod, a single reagent was added to 100 µl of CSF or urine and the absorbancy of the reaction mixture was read after mixing. The absorbancy of the reaction mixture was linearly proportional to the amount of albumin present in the sample (up to 300 mg/dl). The coefficient of variation of within run precision was 5.94, 3.81% for high and normal values of CSF and 3.24, 4.00% for high and normal values of urine. The coeffient of variation of day to day precision was 8.80, 5.69% for high and normal value of CSF and 4.58, 5.81% for high and normal values of urine. The recovery data for albumin is 92-110%. The determination of albumin by electrophoresis and immunochemistry are expensive technique. The present method is simple and give good accuracy and precision, wide range linearity, and needs only a small amount of simple. It id suitable for small laboratories because it requires only simple quipment.

Evaluation of urine calcium determination by qualitative and quantitative method.

Urine calcium were determined in 200 patients of outer patient department, Siriraj Hospital by the qualitative method (Sulkowitch) and the quantitative method (Autoanalyzer). The methods were compared. The qualitative method resulted as negative, 1+, 2+, 3+, and 4+ the quantitative method as 0.5, 1.5, 3.6, 13.2 and 20.1 mg/dl respectively. The ranges were 0.2-0.7, 0.8-2.6, 2.6-5.9, 6.8-16.7 and 16.2-38.6 mg/dl respectively. This paper suggested that the qualitative analysis is simple and can be used as primary test for routine urine calcium screening.

Low cost pregnancy test and HCG stability.

A modified technique for pregnancy slide test (Roche) using hypodermic needles No.23 as droppers for urine and needles No.25 as droppers for antiserum and for human chorionic gonadotropin (HCH)-latex suspension was reported. One hundred positive-and 26 negative-urine specimens by regular Roche pregnancy slide test were used as study samples. The results showed that the modified technique was compatible with the standard method regarding sensitivity and reliability. The modified technique, is however, 6.76 times less expensive than the standard method. The authors recommend this new technique to be used in hospital laboratories and clinics as routine laboratory test. In this study if was also found that the urine specimens that kept at room temperature for up to one year the HCG hormone remained active though the number of positively was reduced if kept 1ฝ year and 2 years to 98.41 and 93.65 percent respectively. Urine specimens that preserved in refrigerator freezer remained positive for HCG hormone for up to 2 years.