ABSTRACT
Development of a novel in vivo lung perfusion(IVLP)procedure allows localized delivery of high-dose doxorubicin(DOX)for targeting residual micrometastatic disease in the lungs.However,DOX delivery via IVLP requires careful monitoring of drug level to ensure tissue concentrations of this agent remain in the therapeutic window.A small dimension nitinol wire coated with a sorbent of biocompatible morphology(Bio-SPME)has been clinically evaluated for in vivo lung tissue extraction and determina-tion of DOX and its key metabolites.The in vivo Bio-SPME-IVLP experiments were performed on pig model over various(150 and 225 mg/m2)drug doses,and during human clinical trial.Two patients with metastatic osteosarcoma were treated with a single 5 and 7 μg/mL(respectively)dose of DOX during a 3-h IVLP.In both pig and human cases,DOX tissue levels presented similar trends during IVLP.Human lung tissue concentrations of drug ranged between 15 and 293 μg/g over the course of the IVLP procedure.In addition to DOX levels,Bio-SPME followed by liquid chromatography-mass spectrometry analysis generated 64 metabolic features during endogenous metabolite screening,providing information about lung status during drug administration.Real-time monitoring of DOX levels in the lungs can be per-formed effectively throughout the IVLP procedure by in vivo Bio-SPME chemical biopsy approach.Bio-SPME also extracted various endogenous molecules,thus providing a real-time snapshot of the physi-ology of the cells,which might assist in the tailoring of personalized treatment strategy.
ABSTRACT
Introduccion: Lippia alba (Mill) N.E. Brown es una planta aromatica perteneciente a la familia Verbenaceae, ampliamente usada en Suramerica y Norteamerica como infusion contra la hipertension, problemas digestivos, nauseas y resfriados. Objetivo: En el presente estudio, se evaluo la actividad antiviral in vitro frente al Herpesvirus Humano Tipo 1 (HSV-1), de veinte aceites esenciales de L. alba y, diez de sus componentes mayoritarios. Metodologia: La actividad antiviral in vitro fue evaluada empleando la t¨¦cnica modificada, de titulacion del punto final (EPTT). Los aceites esenciales, de plantas de L. alba recolectadas de diferentes regiones del pais, fueron obtenidos por hidrodestilacion asistida con radiacion de microondas (MWHD). Se determino por cromatografia de gases¨Cespectrometria de masas (GC-MS), la composicion quimica, de los veinte aceites esenciales de L. alba, identificandose dos quimiotipos: citral y carvona. Resultados y conclusiones: Los aceites quimiotipo carvona, BC1 y CA2, mostraron actividad antiherpetica in vitro, moderada sobre monocapa de celulas HeLa infectadas, con valores de Rf de 1x101,5, en concentraciones, de 250¦Ìg/mL y 125¦Ìg/mL, respectivamente. Los controles positivos, sulfato de heparina y aciclovir, redujeron el titulo viral con valores de Rf, en orden, de 1x102 y 1x104. Ninguno de los monoterpenos evaluados mostro actividad contra el HSV-1.
Introduction: Lippia alba (Mill.) N.E. Brown is an aromatic shrub belonging to the Verbenaceae family, which is widely used all over South and Central America as an infusion against, hypertension, digestive troubles, nausea and cold. The antiviral activity in vitro against Herpesvirus 1, Human (HSV-1) of twenty essential oils of L. alba and ten of its components were evaluated. Methodology: The antiviral activity was determined using a modified end-point titration technique (EPPT). The essential oils from L. alba collected in different regions of Colombia were obtained by microwave-assisted hydrodistillation (MWHD). Their chemical compositions were determined by GC and GC/MS. Two chemotypes were distinguished, characterized by carvone and citral as main constituents. Results and conclusions: Carvone chemotype oils BC1 and CA2 were found to be moderate antiviral activity over infected confluent monolayers of HeLa cells with Rf value of 1x101,5 at a concentration of 250¦Ìg/mL and 125¦Ìg/mL, respectively. Heparin sodium salt and acyclovir were used as positive controls and showed Rf values of 1x102 and 1x104, respectively. None of monoterpenes tested showed antiviral activity against HSV-1.
Subject(s)
Alphaherpesvirinae , Infections , Monoterpenes , Public HealthABSTRACT
En el presente artículo de revisión se describen las múltiples aplicaciones de los procedimientos isotópicos en el diagnóstico y seguimiento de diferentes entidades patológicas en el paciente pediátrico, haciendo además referencia a las técnicas de exámen, dosis administradas, ventajas y limitaciones de este importante método diagnóstico