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1.
Arch. med. res ; 28(1): 5-9, mar. 1997. ilus
Article in English | LILACS | ID: lil-225190

ABSTRACT

We report a study on the DNA organization in Entamoeba histolytica using a ribosomal DNA probe. The rDNA genes were found forming mers which were separated in a typical ladder pattern by pulse field electrophoresis. DNA rosette structures were visualized through electron microscopy in DNA eluted from bands recognized by the ribosomal probe. The in situ hybridization experiments using a DNA probe suggested that the rDNA genes are portioned between the nucleus and a cytoplasmic structure. These findings provide new data on DNA organization in E. histolytica and open the question concerning the presence of a novel organelle in this eukaryotic parasite


Subject(s)
Animals , DNA, Ribosomal/genetics , DNA, Ribosomal/ultrastructure , Cytoplasm/chemistry , DNA, Protozoan/genetics , DNA, Protozoan/ultrastructure , Entamoeba histolytica/genetics , Entamoeba histolytica/ultrastructure , In Situ Hybridization
3.
Rev. cuba. med ; 25(2): 182-6, feb. 1986. tab
Article in Spanish | LILACS | ID: lil-44349

ABSTRACT

Se realizaron 40 mediciones de siluetas ventriculares correspondientes a 20 ventriculografías realizadas en forma consecutiva. Los procedimientos utilizados fueron la planimetría, el recuento visual de los milímetros cuadrados incluidos en la silueta ventricular y el uso de tableta gráfica y lápiz digitalizador. Este último procedimiento se realizó en 2 centros distintos. El análisis de varianza de 2 clasificaciones no encontró diferencias significativas entre los distintos procedimientos por lo que éstos pueden utilizarse indistintamente para los cálculos de la ventriculografía cuantitativa


Subject(s)
Humans , Heart Ventricles
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