Preliminary study of metabonomcs on aqueous extract of Evodia rutaecarpa in sprague-dawley rats / 中国中药杂志

<p><b>OBJECTIVE</b>To study the change of endogenous metabolites of SD rats administrated of aqueous extract of Evodiae rutaecarpa.</p><p><b>METHOD</b>Six SD rats had been successively administrated aqueous extract of E. rutaecarpa (0.3857 g x kg(-1)) for 33 days. An agilent 1200 6410 triplequadrupole mass spectrometer was used for the analysis of endogenous metabolites in rat urine samples. These data was analyzed by the principal component analysis (PCA) and PLS-DA using the SIMCA-P 10.0 software.</p><p><b>RESULT</b>The significant difference in metabolic profiles between the control group and the dosed group was well observed by PCA of the MS data.</p><p><b>CONCLUSION</b>The E. rulaecarpa has changed the endogenous metabolites of SD rats. This work can provide the base for the further research on the interpretation of drug property of E. rulaecarpa.</p>

Animal models for traumatic brain injury and its application in experimental therapeutics / 中国药理学通报

Traumatic brain injury(TBI), also known as intracranial injury or head trauma, specifically refers to the brain tissue damage caused by trauma.Currently the mechanism of TBI and repair therapy after nerve injury become a hotspot in brain research.Duplicating animal models plays a significant role in promoting experimental therapeutics of TBI.This review systematically describes the progress in animal models for TBI including impact brain injury, nonimpact acceleration head injury and blast(explosion)wave-induced neurotrauma, which have been established at home and abroad.Based upon the aforementioned models, some relevant applications in experimental therapeutics are simultaneously enumerated.Hopefully all these information provides scientific guidance for the pharmacodynamic screening of potential neuroprotective drugs.

Effect on factors of energy metabolism in rats skeletal muscle by cold traditional Chinese medicine / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the effect of six cold traditional Chinese medicine on the energy metabolism factors in rats skeletal muscle.</p><p><b>METHOD</b>The activity of Na(+)-K(+) -ATPase, Ca(2+) -ATPase and succinate dehydrogenase (SDH), the content of muscle glycogen, and the mRNA expression of skeletal muscle uncoupling protein 3 (UCP3) were measured after rats having been administrated with water extracts of Radix Sophorae Flavescentis, Fructus Gardeniae, Cortex Phellodendri, Radix Scutellariae, Rhizoma Coptidis, and Radix Gentianae respectively at the dose of 6.0, 7.0, 8.4, 6.0, 7.0, 4.0 g x kg(-1) for 30 days.</p><p><b>RESULT</b>The activity of Na(+) -K(+) -ATPase has been depressed significantly and the content of skeletal muscle glycogen has been increased remarkably by six cold traditional Chinese medicine. The decreased tendency has been found on activity of Ca(2+) -ATPase and SDH, only the Radix scutellariaeg group decreased the Ca(2+) -ATPase activity significantly (P < 0.05), the SDH activity was decreased high significantly by Radix scutellariae, Cortex Phellodendri, Radix Gentianae and significantly by Rhizoma Coptidis. The mRNA expression of UCP3 has been decreased high significantly by all five cold traditional Chinese medicine except Cortex Phellodendri group with the decreased tendency of UCP3 mRNA expression.</p><p><b>CONCLUSION</b>The cold traditional Chinese medicine has the significant effects on the skeletal muscle energy metabolism by decreasing the utlization of the glucose and the activity of mitochondria SDH to reduce the production of ATP, and depressing the activity of Na(+)-K(+) -ATPase and Ca(2+) -ATPase to cut down the consumption of ATP, by decreasing the mRNA expression of UCP3 to decrease the heat production.</p>

Study of hepatic energy metabolism on rats by six cold property traditional Chinese medicines / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the common rule hepatic energy metabolism on rats by six cold property of traditional Chinese medicines (TCM).</p><p><b>METHOD</b>The activities of Na+ - K+ - ATPase, Ca2+ - ATPase and SDH, the content of hepatic glycogen, and the mRNA expression of hepatic uncoupling protein 2 (UCP2) were measured after the rats and been administrated with water extracts of Radix Sophorae Flavescentis, Fructus Gardeniae, Cortex Phellodendri, Radix Scutellariae, Rhizoma Coptidis, Radix Gentianae respectively at 6.0, 7.0, 8.4, 6.0, 7.0, 4.0 g x kg(-1) doses for 30 days, twice a day.</p><p><b>RESULT</b>The activities of Na+ - K+ - ATPase, Ca2+ - ATPase and SDH were depressed significantly by six cold property TCM. The decreased tendency of SDH was observed by six cold property of TCM, while statistical significance was obtained in Cortex Phellodendri, Radix Scutellariae, Rhizoma Coptidis, Radix Gentianae. The increased tendency of hepatic glycogen content was found by six cold property TCM, while statistical significance was not obtained in six groups. The decreased tendency of the mRNA expression of UCP2 was found by six cold property TCM, while statistical significance was obtained in Radix Sophorae Flavescentis, Cortex Phellodendri, and Radix Scutellariae groups.</p><p><b>CONCLUSION</b>The six cold property TCM have the good effects on hepatic energy metabolism by decreasing the activity of mitochondria SDH to reduce the production of ATP by decreasing the activities of Na+ - K+ - ATPase, Ca2+ - ATPase to cut down the consumption of ATP, by decreasing the mRNA expression of hepatic UCP2 to decrease the heat production.</p>

Study of metabonomics on pharmacological action appraisal Rhizoma coptidis in rats / 中国中药杂志

<p><b>OBJECTIVE</b>HPLC-MS/MS-based metabonomics method was used to find the possible biomarker of Rhizoma Coptidis in rat urine.</p><p><b>METHOD</b>Sprague-Dawley rats were successively administrated 7 g x kg(-1) aqueous extract of Rhizoma Coptidis for 30 days, urine were collected by metabolism cages and detected by using the HPLC-MS-MS. All dates were analyzed by the principal component analysis (PCA) through using the SIMCA-P 10.0 software.</p><p><b>RESULT</b>The PCA demonstrated that the metabolome between treated group and control group had difference in rat urine sample after of 22 days administrated, for treated group 169 kinds of biomarkers were found including oxalacetic acid, malic acid, 2-ketoglutaric acid, NE, arachidonic acid, 5-HIAA and other compounds, the result was consistent with pharmacological effects of R. coptidis, such as antiinflammatory, inhibiting biosynthesis of CA biosynthesis, anticentral nerve and energy metabolism inhibition.</p><p><b>CONCLUSION</b>Metabonomics may be available in pharmacological action evaluation of drugs.</p>

Duplication of animal models for spinal cord injury and its application in experimental therapeutics / 中国药理学通报

The mechanism of spinal cord injury and repair therapy after nerve injury is currently a hotspot of neuroscience research.Duplicating animal models plays a key role in experimental therapeutics of spinal cord injury.This review systematically describes the progress in animal models for spinal cord injury including contusion, compression, transection, ischemic,distraction and chemical-mediated injury,which have been established at home and abroad.Based upon the aforementioned models,some applications in experimental therapeutics are simultaneously enumerated.All these information provides scientific guidance for the experimental novel drugs′screening.

Biocompatibility test of polymethylmethacrylate denture base resin containing silver-supported antimicrobial agent STR-1 at nanometer level / 北京大学学报(医学版)

Objective: To evaluate the biocompatibility of polymethylmethacrylate(PMMA) denture base resin containing silver-supported antimicrobial agent STR-1 of nanometer level in vitro. Methods: According to the national standards for biological evaluation of dental materials, the cytotoxicity of denture base resin containing STR-1 at concentrations of 5 g/L and 10 g/L was examined by molecular filtrating method, and the hemolysis of STR-1, denture base resin containing STR-1 at concentrations of 5 g/L and 10 g/L was also surveyed. Results: The control denture base resin without containing STR-1 and the denture base resins containing STR-1 at concentrations of 5 g/L and 10 g/L were not cytotoxic to L929 cells. Two hours and 24 hours after cell culturing, the filter membranes of the control and experimental groups were stained evenly with blue color. The staining intensity was not decreased and the fading areas were 0 mm~2 during the culturing. The cytotoxicity grades were 0. The hemolysis rates of the antimicrobial agent STR-1 and the denture base resins containing STR-1 at concentrations of 5 g/L and 10 g/L were 1.7%, 3.5% and 3.7% respectively. They were less than the national guild standard 5% which represent no hemolysis. Conclusion: The PMMA denture base resins containing silver-supported antimicrobial agents STR-1 of nanometer level at concentrations of 5 g/L and 10 g/L exhibit good biocompatibility.

Durability of in vitro antimicrobial effect of polymethylmethacrylate denture base containing silver-supported antimicrobial agent of nanometer level / 实用口腔医学杂志

Objective: To study the durability of the antimicrobial effect of polymethylmethacrylate (PMMA) denture base containing silver-supported antimicrobial agents of nanometer level against Streptococcus mutans and Candida albicans in vitro. Methods:The antimicrobial PMMA resin bases with the antimicrobial agent STR-1 at concentration of 5 mg/ml were prepared. Then the samples were divided into 4 groups: positive control group, the group immersed in distilled water at 57 ℃ for 14 days, the group irradiated by ultraviolet for 8 hours, and the group irradiated by ultraviolet for 8 hours after immersed in distilled water at 57 ℃ for 14 days. Then the inhibition ratios of the 4 groups against Streptococcus mutans and Candida albicans in vitro were tested. Results:The inhibition ratios of the 4 groups against Streptococcus mutans were 99%, 96%, 98%, and 90% in turn. The inhibition ratios of the 4 groups against Candida albicans were 91%, 82%, 90%, and 80% respectively. Conclusion: The antimicrobial effect of PMMA denture base containing silver-supported antimicrobial agents STR-1 of nanometer level at concentration of 5 mg/ml against Streptococcus mutans and Candida albicans in vitro is durable.

In vitro antimicrobial effect of polymethylmethacrylate denture base with silver-containing antimicrobial agents of nanometer level / 实用口腔医学杂志

Objective:To study the antimicrobial effect of polymethylmethacrylate (PMMA) denture base with silver-containing antimicrobial agents of nanometer level against Streptococcus mutans and Candida albicans in vitro. Methods:Minimum inhibitory concentration (MIC) of the silver-containing antimicrobial agent against S. mutans and C. albicans was examined. According to the MIC, 4 concentrations(1,2,5 and 10 mg/ml) of the agent were selected for the preparation of antimicrobial PMMA resin base. Then, the antimicrobial effect of the resin base was examined by in vitro bacteria culture and the most probable number(MPN) counting. Results:The MICs of the agent against S. mutans and C. albicans were 0.1 mg/ml and 1 mg/ml respectively. With the agent at 1, 2, 5 and 10 mg/ml, the inhibition ratios of the base against S.mutans were 67.4%,71.3%,99.0 and 99.5% respectively, that against C. albicans were 25.8%,54.8%,90.3% and 93.0%, respectively. Conclusion:The polymethylmethacrylate resin base with silver-containing antimicrobial agent of nanometer level at 5 mg/ml has ideal antimicrobial activity against S. mutans and C. albicans.

Study on extracting process of Fumai Decoction / 中草药

Object To optimize the extraction conditions of Fumai Decoction. Methods Uniform design test was used, and the contents of glycyrrhizinic acid and ginsenoside Rb 1 in extracted solvent were determined by HPLC. Results The ethanol volume and the extracting temperature in the uniform test impacted the test remakably. The best extraction process of Fumai Decoction as follows: the raw drugs were extracted by 10 times of 50% ethanol at 60 ℃ for 4 hours. Conclusion Glycyrrhetinic acid and ginsenoside Rb 1 contents in Fumai Decoction are higher according to the above extraction process.