ABSTRACT
Avaliar o efeito do suramin na migração, proliferação e formação de tubo vascular em células endoteliais coroidianas (CECs) in vitro e em neovascularização coroidiana (NC) in vivo. Foi avaliada a migração através do experimento de Boyden Chamber. Foi avaliada a proliferação através do experimento MTT. Foi avaliada a formação de tubo vascular através do experimento gel colágeno 3D. As CECs foram estimuladas por fatores de crescimento (FC) e tratadas com suramin.O efeito sistêmico do suramin foi avaliado em NC induzidos por laser em olhos de ratos. O suramin inibiu a migração, proliferação e formação de tubo vascular estimulada por FC de forma dose dependente / This study evaluated the effects of suramin on choroidal endothelial cell (CEC) migration, proliferation and tube formation in vitro and choroidal neovascularization (CNV) in vivo. Migration was evaluated using Boyden chamber assay. Proliferation was evaluated by an MTT assay. Tube formation was evaluated using a 3D-tube formation assay. CECs were stimulated by growth factor (GF) treated with suramin. The effect of systemic administration of Suramin was evaluated on laser induced CNV in rats eyes. Suramin inhibited CEC migration, proliferation, and tube formation induced by GF in a dose dependent manner. CNV in rats was inhibited by systemic administration of Suramin 30mg/Kg. These studies indicate that suramin inhibits Angiogenesis in vitro and in vivo...