ABSTRACT
Microdeletions in Yq are associated with defects in spermatogenesis, while those in the AZF region are considered critical for germ cell development. We examined microdeletions in the Y chromosomes of patients attended at the Laboratory of Human Reproduction of the Clinical Hospital of the Federal University of Goiás as part of a screening of patients who plan to undergo assisted reproduction. Analysis was made of the AZF region of the Y chromosome in men who had altered spermograms to detect possible microdeletions in Yq. Twenty-three patients with azoospermia and 40 with severe oligozoospermia were analyzed by PCR for the detection of six sequence-tagged sites: sY84 and sY86 for AZFa, sY127 and sY134 for AZFb, and sY254 and sY255 for AZFc. Microdeletions were detected in 28 patients, including 10 azoospermics and 18 severe oligozoospermics. The patients with azoospermia had 43.4% of their microdeletions in the AZFa region, 8.6% in the AZFb region and 17.4% in the AZFc region. In the severe oligozoospermics, 40% were in the AZFa region, 5% in the AZFb region and 5% in the AZFc region. We conclude that microdeletions can be the cause of idiopathic male infertility, supporting conclusions from previous studies.
Subject(s)
Humans , Male , Chromosomes, Human, Y/ultrastructure , Chromosome Deletion , Gene Deletion , Infertility, Male/genetics , Azoospermia/genetics , Brazil , Germ Cells/metabolism , Spermatogenesis , Fertility , Polymerase Chain ReactionABSTRACT
Dois grupos de 25 pacientes cada um, com diagnostico de salpingite aguda uni ou bilateral, foram incluidos neste estudo duplo-cego e randomizado. O 1o. grupo foi tratado com ampicilina e naproxen sodico, um antiinflamatorio nao hormonal, de uso oral, durante 10 dias, enquanto que o 2o.grupo foi tratado com ampicilina e placebo pelo mesmo periodo. O objetivo do estudo foi verificar a eficacia e a diferenca entre os dois grupos. Os dados finais mostram que em favor do grupo antibiotico mais antiinflamatorio houve melhores resultados, tanto em relacao a eficacia, quanto a diminuicao do VHS e da leucocitose. A tolerancia a medicacao nos dois grupos foi satisfatoria, nao ocorrendo efeitos secundarios de importancia. Os AA.concluem que, apesar do pequeno numero de casos estudados, a associacao naproxen sodico a antibioticoterapia e uma tentativa valida no tratamento da doenca inflamatoria pelvica e, nesse estudo, mostrou-se superior ao grupo antibiotico-placebo