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Rev. Inst. Adolfo Lutz ; 66(2): 158-163, maio-ago. 2007. tab, graf
Article in Portuguese | LILACS, SES-SP, SESSP-CTDPROD, SES-SP, SESSP-IALPROD, SES-SP | ID: lil-477258

ABSTRACT

Santa Catarina, foram analisados quanto ao conteúdo total de polifenóis e capacidade antioxidante. Foram utilizados sistemas solventes aquosos a 50% (v/v) de metanol, etanol e acetona acidificados (HCl 0,1%). O conteúdo de fenólicos totais foi determinado pela técnica de Folin-Ciocalteu e a atividade antioxidante por meio de técnicas de seqüestro de radicais livres ABTS e DPPH e pelo método do ß-caroteno/ácido linoléico. A acetona apresentou maior conteúdo de polifenóis totais, atingindo em peso seco, 7852mg GAE/100g na variedade Pinot Noir e 5334mg GAE/100g na variedade Regente. A atividade antioxidante média das variedades Pinot Noir e Regente foi, respectivamente, de 477 e 419μMol TEAC/g pela técnica ABTS e 480 e 479μMol TEAC/g pela técnica DPPH. No ensaio da co-oxidação do ß−caroteno/ácido linoléico os extratos etanólicos apresentaram maior sinergismo quando associados ao BHT, com 92,6% e 90,9% de inibição da oxidação com 200μL para a variedade Regente e Pinot Noir, semelhante ao BHT com 200μL, apresentando 91,7% de inibição da oxidação. Os extratos de bagaço de uva demonstraram potencial antioxidante, atuando como inibidores de radicais livres ou em sinergismo com o antioxidante sintético.


Grape (Vitis vinifera) bagasse from Pinot Noir and Regente varieties, collected in Videira - Santa Catarina, were evaluated in order to determine the total polyphenol contents and antioxidant capacity. Acidified (HCl 0.1%) aqueous solvent systems of 50% (v/v) methanol, ethanol, and acetone were used. Total polyphenol contents were determined by Folin-Ciocalteu technique, and antioxidant activity was assessed by means of ABTS, DPPH, and β-carotene/linoleic acid techniques. Acetone presented the highest total polyphenol contents, reaching in a dry matter the concentrations of 7.852mg GAE/100g in Pinot Noir variety and 5.334mg GAE/100g in Regente variety. The average antioxidant activity of Pinot Noir and Regente varieties was of 477 and 419μMol TEAC/g by means of ABTS technique, respectively, and 480 and 479μMol TEAC/g, respectively when these samples were analyzed by DPPH technique. From β-carotene/linoleic acid technique analysis, it was observed that the ethanolic extracts showed higher synergism when associated to BHT, presenting 92.6% and 90.9% of oxidation inhibition with 200μL for Regente and Pinot Noir varieties, which were similar to BHT with 200μL, presenting 91.7% of oxidation inhibition. Extracts from grape bagasse demonstrated a potential antioxidant activity, functioning as inhibitors of free-radicals, or in synergism with a synthetic antioxidant.


Subject(s)
Antioxidants , Vitis
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