ABSTRACT
Objective: This study intended to observe the effects of methoxyamine [Mx] on cytotoxic effects and DNA damage caused by 5-Fluorouracil [5-FU] in combination with gamma radiation in a human colon cancer cell line, HT29
Materials and Methods: In this experimental study, HT29 cells were cultured as a monolayer and treated with different concentrations of 5-FU along with 1 mM Mx for 24 hours. Next, the cells were irradiated with 2 Gy gamma radiation. After the treatments, we assessed for DNA damage, cytotoxicity, and viability by alkaline comet, clonogenic survival, and trypan blue dye exclusion assays
Results: Cytotoxicity and DNA damage increased with increasing 5-FU concentration. The 1 mM Mx concentration had no significant effect on cytotoxicity and DNA damage from 5-FU; however, it increased the cytotoxic and genotoxic effects of different concentrations of 5-FU when used in combination with 2 Gy gamma radiation
Conclusion: Mx combined with 5-FU enhanced the radiosensitivity of colon cancer cells